	   SnapGene      )¬acgcgtgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattgccgcattgcagagatattgtatttaagtgcctagctcgatacaataaacgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacctgaaagcgaaagggaaaccagagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccactgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcctcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagattggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtATCGGTtaacttttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttatcgatactagtattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtTtatataagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagattctagagctagcCTGATACGAACTCGgaattcgccaccatgcagaggctcatgatgctcctcgccacatcgggcgcctgcctgggcctgctggcagtggcagcagtggcagcagcaggtgctaaccctgcccaacgggacacccacagcctgctgcccacccaccggcgccaaaagagagattggatttggaaccagatgcacattgatgaagagaaaaacacctcacttccccatcatgtaggcaagatcaagtcaagcgtgagtcgcaagaatgccaagtacctgctcaaaggagaatatgtgggcaaggtcttccgggtcgatgcagagacaggagacgtgttcgccattgagaggctggaccgggagaatatctcagagtaccacctcactgctgtcattgtggacaaggacactggcgaaaacctggagactccttccagcttcaccatcaaagttcatgacgtgaacgacaactggcctgtgttcacgcatcggttgttcaatgcgtccgtgcctgagtcgtcggctgtggggacctcagtcatctctgtgacagcagtggatgcagacgaccccactgtgggagaccacgcctctgtcatgtaccaaatcctgaaggggaaagagtattttgccatcgataattctggacgtattatcacaataacgaaaagcttggaccgagagaagcaggccaggtatgagatcgtggtggaagcgcgagatgcccagggcctccggggggactcgggcacggccaccgtgctggtcactctgcaagacatcaatgacaacttccccttcttcacccagaccaagtacacatttgtcgtgcctgaagacacccgtgtgggcacctctgtgggctctctgtttgttgaggacccagatgagccccagaaccggatgaccaagtacagcatcttgcggggcgactaccaggacgctttcaccattgagacaaaccccgcccacaacgagggcatcatcaagcccatgaagcctctggattatgaatacatccagcaatacagcttcatcgtcgaggccacagaccccaccatcgacctccgatacatgagccctcccgcgggaaacagagcccaggtcattatcaacatcacagatgtggacgagccccccattttccagcagcctttctaccacttccagctgaaggaaaaccagaagaagcctctgattggcacagtgctggccatggaccctgatgcggctaggcatagcattggatactccatccgcaggaccagtgacaagggccagttcttccgagtcacaaaaaagggggacatttacaatgagaaagaactggacagagaagtctacccctggtataacctgactgtggaggccaaagaactggattccactggaacccccacaggaaaagaatccattgtgcaagtccacattgaagttttggatgagaatgacaatgccccggagtttgccaagccctaccagcccaaagtgtgtgagaacgctgtccatggccagctggtcctgcagatctccgcaatagacaaggacataacaccacgaaacgtgaagttcaaattcatcttgaatactgagaacaactttaccctcacggataatcacgataacacggccaacatcacagtcaagtatgggcagtttgaccgggagcataccaaggtccacttcctacccgtggtcatctcagacaatgggatgccaagtcgcacgggcaccagcacgctgaccgtggccgtgtgcaagtgcaacgagcagggcgagttcaccttctgcgaggatatggccgcccaggtgggcgtgagcatccaggcagtggtagccatcttactctgcatcctcaccatcacagtgatcaccctgctcatcttcctgcggcggcggctccggaagcaggcccgcgcgcacggcaagagcgtgccggagatccacgagcagctggtcacctacgacgaggagggcggcggcgagatggacaccaccagctacgatgtgtcggtgctcaactcggtgcgccgcggcggggccaagcccccgcggcccgcgctggacgcccggccttccctctatgcgcaggtgcagaagccaccgaggcacgcgcctggggcacacggagggcccggggagatggcagccatgatcgaggtgaagaaggacgaggcggaccacgacggcgacggccccccctacgacacgctgcacatctacggctacgagggctccgagtccatagccgagtccctcagctccctgggcaccgactcatccgactctgacgtggattacgacttccttaacgactggggacccaggtttaagatgctggctgagctgtacggctcggacccccgggaggagctgctgtatCTCGAGTACCCATACGACGTACCTGATTATGCAACTTATCCTTATGATGTACCTGATTATGCTAGCTATCCTTATGATGTCCCAGACTACGCATAGggatccCTGCGATATCCAGCgcggccgcGaaggatctgcgatcgctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaacgggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacagctgaagcttcgaggggctcgcatctctccttcacgcgcccgccgccctacctgaggccgccatccacgccggttgagtcgcgttctgccgcctcccgcctgtggtgcctcctgaactgcgtccgccgtctaggtaagtttaaagctcaggtcgagaccgggcctttgtccggcgctcccttggagcctacctagactcagccggctctccacgctttgcctgaccctgcttgctcaactctacgtctttgtttcgttttctgttctgcgccgttacagatccaagctgtgaccggcgcctacgctagacgccaccatggagagcgacgagagcggcctgcccgccatggagatcgagtgccgcatcaccggcaccctgaacggcgtggagttcgagctggtgggcggcggagagggcacccccaagcagggccgcatgaccaacaagatgaagagcaccaaaggcgccctgaccttcagcccctacctgctgagccacgtgatgggctacggcttctaccacttcggcacctaccccagcggctacgagaaccccttcctgcacgccatcaacaacggcggctacaccaacacccgcatcgagaagtacgaggacggcggcgtgctgcacgtgagcttcagctaccgctacgaggccggccgcgtgatcggcgacttcaaggtggtgggcaccggcttccccgaggacagcgtgatcttcaccgacaagatcatccgcagcaacgccaccgtggagcacctgcaccccatgggcgataacgtgctggtgggcagcttcgcccgcaccttcagcctgcgcgacggcggctactacagcttcgtggtggacagccacatgcacttcaagagcgccatccaccccagcatcctgcagaacgggggccccatgttcgccttccgccgcgtggaggagctgcacagcaacaccgagctgggcatcgtggagtaccagcacgccttcaagacccccatcgccttcgccagatcccgcgctcagtcgtccaattctgccgtggacggcaccgccggacccggctccaccggatctcgcgagggcagaggaagtcttctaacatgcggtgacgtggaggagaatcccggccctatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtctccgccttcgccctcagacgagtcggatctccctttggccgcctccccgcctggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaaaataagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggctctagctatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctagacttttgcagagacggcccaaattcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgccaagctg  YY   9   :      <   =   5   (   /   3            A   5   4   >   6   G   (   7   =   1   !   %   !         0   .   /      1   B   &   $   5                $      $          "         ;   !   "   7   E   9   #   .   C   "   *   ;   4   ?   B   <   /   2      4   ;   E   2   ,   .   (   %      $   >   ;   (   6   8   *   1   7   U   +         *   '         &   (   #   "   '      $      '         *   )         !   /   (   *   *   ,      )   1   /   %      %         $   +   8   *   2   6   @   2   /   -   (      ,   2      "      !   1   3   7                  #               #      "          #   (      $   )      "   +      (      4   $   "   *      /   $         '   -   )   '   1   4      %   '   &   *   )   )   +      &   I   7         &   .         "   /   +   (   T   -   A   ,   E   =   -   !   -   (      $   =   (   (       H   K   /   ,   :            %         ,   ;      3   1   4   .   #      O   <   '   1   A   E      5   .   9   4   *   =        9 ûQR}~Ú%§!"#0ý
TÎÏÐ‹ã4Fûþÿ ,bc¹./à^Æï÷øù¼) « _#›&­'›(T)U): ¬½Sf«î½-K�{¨1¥1M�m	T
þ
0UÑ.mœGü+›6d~ð)úSgü* ¬ ©!„#T$ó$^&+'j(")V) – é 8C&$$
-^˜—Ó¬á4½`#'s'®'Š(”(p){)< ü¢`°Ûöõ{,	8	
ÆØ>JŒ¿è`­Ú£ä5@¥ÉÙºK>JY¤[¯Á0fÞF½¦²_ÇÔ? ñ!X$´$œ&3'~(œ(')4)= I 	#.T¬­°³Æõ4L[ˆ=Í2�¿
5
U
”
1B¢n =H,/ebê/E�KNoÿþvÈÍñT1=+ U$H(k(ï(5 i ˆ �¾g€]r.Uÿ0Okuè»2ÿ£	V´/…[g]7åX¡ñ hÚ.¦ý· Ü b!ª!ß"p#°$ô$�&','<'#)( ¡ 
G„|ôû�	sÚËQ�8�ïäœ»Óuµœ­ �!h"?#…# #_&¬&†'c(W)/ ¦ ­Öï¾Ž©¦Nn	O
|
‚
ÿ
s�Ò\˜U¯Ûö²ýfoý#mú·Ä†Úô@Á*RûD!³"3 f q 9ÊÙŒZ	±þÊÖ]Ì$_;UX¾÷÷&Îéâ6Ma8æ¾3!Ù!‚"˜"#9%&'"(+(‹(•(Ô(/) * t jx‘ Eþ%¤2.0‘¸µ} ’ Ì!""<"a"w"¾"ë"#L%q) ó rD'Iá“Ø
™ó;�Z>H�3 š #a%–%Õ%t'¯'|) — ê 
%
ê=ÛzŒ‘A˜Ôï­5hh ß!a#Œ#è#Å$£%N(A  öý aºö|ƒ-	‹	‰
�ÛQT¤§ªàly'WÒ*?B{íJ°ãáìd’Ð3ž0 @ v � õ 6!U!²!"q%=&S&�&4'ÿ'(‘()()M)d)5 Œ ï£Üí÷w´ç>A	G	P	Ø	
Ù?ÀžÀa®q)6NÊÚKº\Âg»ßÉ§³+`ÕÓT"ú"$1%Û%'®(4 Ü&Ý±ù@	K
÷
K»W�¦K»w¥«ÉðbæÊ¾BäÈÍm^²é4p !ò!¦"Y$Ž$Q%�(¡()> xÈ™~³9	Æ
ŸÇÞ©éEÓJÂõdåóA²ãLúZ:>1G#.F\oˆÓCbu¨@U Ì × é ›"e$µ$À%(5)›)6 ôù$p®±»®¸>¥‘	I
Z½ÿCíž¡'>[j£-\·áH8kLÜã Î4,
/2>. 1!u"$V$	(G J L Â r/13>UL´Ç“ÎÐÒÔÚÀ•	—	º	
/
6
G
V
•
2•—©¼¨#‘Ä$€­IöfEëô	FÏÿò½$*P, ’#~$.&¼&I(Ã(ð(( 3 A
ö\‰q3ì	b
ï
[ö£Þ¬¸?÷0p2É—Œ&È1"‹"æ"Þ#ü$t%&D&Å(7 
Æ@­v&5M¡êf"6YoÕv‰0Þcíö6q˜%žOa“Ÿ7w×MU¡Ìy Ä !q"¹$	'‚'l(µ(= j ‰ ä+Ú^sÉV¼¸ÁÄÇà·	Q™µ0Ñ‚Ž‘%…^`c¸ø!¯næY=ò'§c!«!“"°"Ï"H#Á#±$À$õ$¶%'f'$)1 N �³¹¿h�…Ñ 1•Ñ3¼	Œ
W‡0†h8h…òÛ}þ¸ 9!à"7#u#Ž& &='�'Ã'Ú'.(Ù(ò(P)! ² WÎ/òPv¿ÖàéPl¼7] 	õ	
X
ám“ ¥ 0&-'E'’'% ª §³ì¢lÚð®ù¤	»
nL\Æ&h…¥&Ñ*¨¢i/¼ Ý þ!q#v&! 0 5c…Ü	ª	´	)
¾ctP¶Žž¯ÂKi‡>k¡U&È&%(d(æ(X) Ä Ý HNl¶ï6}„üôjÌ9Bð¦³¶® N!�!F$O$v$`&‡' b ¢ · qéã™	ž	ÛšRåTrv‰i",#†#”#•$º(g)0 y ý t] àõ·Ý	«ä:‚W�¼ÃÂFv…˜Ô��ÝH¿õ"@#K#S#¡#­#$k%}%«%­&¾&â&+). “ § Ðouû(rÂ8
¶
"t™V›þgv|ßKßñà¸ú9<ÛÞ.Kø+”R!$!f#Ñ'((K(‹)˜)/ œ äðUg¿ƒ�¯ÖÜ?No	1
}
ƒ
¿
÷ 3ªã—ñ÷–³‹n€ûÃÂÛ@!A"´"í#7'ë'8(q( d×§O—
Ó�<ÐÙë÷�nMf�€Êü¯íFZ E!F"6$&1 ø }®‡¨“ª�ÖP
 %4‘]w°Üi¼pþ$GŠÎbƒ„Å‡õžôATSÃCÈ!"€$@&B g r �ž+ý‹ Ëù�E	N	
z
û
.¯²<HS@ŠÀÿâ×%`-<VY›¿õøImµýÑêË³16äØ‹4!°!–"™"#n#Ã$7%& bÌjîÚ¬ê	¹
áÂ	8^<ƒÌŸ#Dtøƒ­(-{ÊÂ $&&'#(,(×($  OQ`‹;†;
Ë
:¾ÀÆÅDÏ9Bçšœ«Öy±Ú!ö!A$:%Ç%5&Œ(–(Õ(5 Y } Ò CS:=—H+[	é
å*¤vÈÎzË×Íu¹'¿ã7Nb;Žž¿ n!ø!ƒ"l$Š%�&Ö&È'Ç(0)[) )   Lá+àø:F<€Õ	zÚ—éì*É!Ü!|"ì"#C$% + m kƒã^_/1¶`³“ â #"="b"à#þ$¹% &Ë&](ë(r)‘)  Ê Hž’!ÿ‹Â]w¥3\8m’Ìàù¢: ~ Í!x"¸"¿"Ö#M%7&–'( u y^&—¸ëˆþ	d
E}ANlF
rK¹/&¾($ ]É(XÙ
Wûô4 $[Ñé¨ˆáï è"/$í$%"%‡%¯%½%F&¹&B'Ž()m)Ž) 5 ô s¿Ís¼?Iƒ› Ö"#ñ#%v%—%É%Ö%u'$ ü	JU�â59”§ÑSˆ¥eš5¦«D5Žr4 ø X!##Ä#%H%ÿ&(})  <E=
°
Í
Ü
îà‰þ²29_<‚ïÒl z!Õ!ã!"�"$$%b%”&Ä&°' \î	&
ñ
ô
'ü¦ÇA}ùB¥~ºÕ�¼1ì™ù i à!>$E%˜(" ‡DÈ+³ã	t
@ë>øW�’Ó™%'pÒõi-!�#<%²%ô%&&V&O(z(„(†) 	  ˜ ë Y�Ö
(+ éð•®�6¤®,"b#é#$â${'Ô'(Y( å
|ÍÜ{®ºŠð$S¨?û.;’—!3"Æ$¤%; þ!O—ß }‰,¹ORU¥¨Þáñm°*ø@CÇîäõ•Øçí9ÑŸ'P`s' 1 t { � €"#”%;&„'a(ˆ(’(¬(Ò(2)! ·÷<aŠ
ºŽÜë«z¤¶ÁïK±ÞÚ7w 7!s"$|$r%>&ž&5'))N)e)"  B÷„è	ã
xü"+BrG]¸}“4ß8 í ö V!®!T&ý& ((‚(é(7 ¡çéb».	Œ	¸ikÙø.®à(ÏX·Ó|Üž)t¶â(Je·ÙÛûE%ä% A b p!³!"Å"2#»$%i&µ'(€())E ¿ ›Ó¨¼(ORd™œŸäx-µèž	B	H	K	Q	T	W	,
œjØáb×a‹OÛwV�Ã'àL´Ôæ<M´ U"X"n"&#)#4#½$Ä%Ü%s&?(n(·(9  # 9 � ð¤.x“½ÌîÄÍøHuÙ	ñ	
óìxëÊÐrZ“�Y¤ÊO¨Y,NUÆ Ñ û""#q$ž$Ë$%Œ%''#')`)# [ º ?ÃÝÓ
‚ÁÁÆq	'–À^  h*Pa[r€ª !2%ä%ê&i'¯(û(. T ëŽûY=?­	Ú@Ÿ©¯µm*7“ËâLs»Ý] ¦ÏTx¼OjG=Öú!$ß%Ø&·'É(á(C áÞr«úE[Î>	}	L
q
ø
ç¦ÊØ¹Žú�25¬É8•»Qc¡C»H»¾Îõg×êo _ q ò Æ!î!ó!"Ì"	#n$¶&ž()J)])§)" '¯ô²TÎì]	¼½žx|Û¦Êñ-3ç®Ó6_¹Î…"§"ø$+%>)* '8EÝa7¨A¢
Œœô²��¬'cÆð¾Ùå‡$+rƒ�?5ñK ˆ!µ!ú#Z$R%&U(¢(; Ù Èç+£		
LCXÍ§ÂÇL¼<Zx” Ë¿Á£tÉ
nW³ŠæC S d „ !'!f!r!À!ç!Ç"ï"i#‡$�$A%\%›%I&ƒ&B(4 _ · Ãö|Ûµ³€žÕäð(t¹[;?D²ÁHWZcäû/Gg¹ÔÝ+«j©£ê …!­$¶$ð$Á% 'g(? yšÅ	$	Ž	ù	A
d ßªêF©ÔK¡æ!R¡çâšá•ÿ”0ov‹‰f™ä
  Í Ø N"["Ò"©$	%%%ù%k&Ê'))F)w)œ)¢)B @  Ô -<ÉgJ»ð0	4	:	y	Ç
ë
:È,Ôm”0QMe†ÕûØ=|Ž+]édÆ®²cv‡¬´Ox‡A¸Á›!"œ"2$×$6)< U£´’jf	€	Áh´Ë$8[�®·ùeëôB€ÏéÑØÿ~‹Ë2 $pyšåý9] ·D� ÁÍ×âVí	!"f$O&(v(/ «¼ú|ª¼¯z¦/SÅ¾çÙ¢?¤š]¸IXÝE¥«Åöfª ƒ#W$³$*'2'r'¬'}(&)S)z)2 H ‡ Õ-	F\q¯²ô3T<º

4
�
[—„ÚfõîŸe.ºlMäu?-0¶ Û C!g"²">#Ÿ#«&'î( p HÉ’Y	
£<yý\#/@TÍ52!Ë!Þ!v"4 ‹ õ%wÜ}¹ì?v²‚	×	J
ž ¿Dc(kâz9[Ï*Ò3?/ Ž Ë Ö è !T!~"$0%p%'þ'
( ))c); K Á qÅó02?Kou%’pÏÑÓÙ é	”	–	¹	.
H
a
Z–5"uÝìõGpâ ¼#%+O- 0!p"Š"å"û$´(Ä(E M *V„²ÐÙÈ¡ñ”¾ÐÕßOÀÃÆ6\ß»	ô	
W
˜m��$Kl’Å%b÷ g¤Ð)§ñ&¤ » ¯"6#G#¿$µ%/&u&œ'Ù'ñ(2 Ã ü s4\Mkµpîß¶â˜	©	Ü	ª½bOã�®J
Á
j„—TˆÜG¾M!+#J#“#¬#E$”$&½&¹(å(, ’ ÷ |ãTf†§‚’ÕÛ>MÁ0
7
–
!3ö2©ð•�FmÞƒó“ÂBÇEQ@"$J(p(. Ñ BNŠ�Ë*–Šií*«D	M	£à½Í7Ö;tÊ�™Õ',°É•"m#$k$Â$Æ%4&€&Ç'Æ(Ÿ)( l KG�*‚ßâ÷]9;Š–·êý	c
\|[@y	q–èë)·"Õ#ß#ý$%¸%&% 4 ¾WûTr4¯
í‡¤dßú413î‡ÑÔ!Œ"Õ"ç"Ã#ð#.$%%%u%¼%E&Ã&l)   †ÕÇâ	í	ð
÷'{V¥­¹ï|ø¤è˜Ñø,!2"á$%&…)$ ¶æ AN–ÿç	·w­ßî÷Æq”
8¶ØOz ­!r"#$º$“%ü&ƒ'`(‡(> 8 S š§-w’ú'NQX›žÂãÌG�J	S	V	›�ëiÅp´Ö`ŠUr�ŸXwNåTZq©Å Ð !W"m"%#(#�$Þ%r&é&
'h'm(¶(; Ø 7Ç®ó`6æ§¢ë¡
‹ó~×�¼Á1{«±;È7&,ÅïPb sôÖV‚>¸nÍ ƒ e!¿!í!î"÷$[%š%A(T(I)¦)( ,fïx	gÊÓ#7Zl“­¶ê Q´dsŠ™×þx\ãNw¢·"Z"Ñ"¨$¬$u(6 G o Š »,vÛE[¹È	

€
Rƒæôxd¹"ySÌZtª>Ñõ� Ê Õ ç ±"=#‚#ž#²$o%ª&')'1'í(%)R)b)8 òƒSjt…
$ð½ÏÔÞNÂÅ5[á“	¸	Û	ó	Yõ1�Jkta	ioƒ‚¦‡Áðº L!?"ä"F#I#&Ø'* k Ð J�ÊÞúá	ü	®
{&z†¼Z¤Ìù:s3ç•(¯+!”"Â#Ô#Á$ %·%»%$&&Æ'k)1 R +6µåM¦_•Wâ¦œæ	Šš¶ÉÒ’¬_é°:ÇT	[óv�Sp¨¶d!¬!ì!Ð"$#ö$’%g'_(t(¥)7 Q 5´º‚DR€Ý	#Ó4Z’	
­
X‰ Ij»99x†ïô€Ro®µŒ ¹ É Ô æ K!ã"<#�#Ó#‘%~&('Å'Q)U O P ‘ijk†‡ˆ”Qzåæ !"2ÆÒ'deqryDW‘	½	¾	�
ˆ‡ýþÿi|îóÜÈ Ó ä å !!á"â"8#9#:#;#v#›#œ#»#¼#Ž%�%�%}&�&¡&'''%'&'''ž'Ÿ'Ä'ø'ù'/(ó(“)+ ’l‰�ç×çc–ò(jszEXwJi}ýÝNbkZ~!¶#½#É#'>' '¨'ú'Ú(ô( å À•Ò{3ÇUf¿	Ž
­1ˆ1–ÿ:!ý"#w#â#£$�&¢&'¤'Û'0(”) lQ×ó3ò.Yxö	à
ý”š™ÃÇEŸÅ ¾#÷#s$'?'“'*  â 2Šè%Ø0`¬Qm½8D
Y
�âîí}OWl•  $%1&Y&c&.'F']'¡'©'û'Û(õ(' ³ Xm�ÂÏè†¦—ž¸áê)^kt{	m¬~Þ	cñ[�Û·#é$è% “ÂódwÀFKn<jþJÞb ¦  !Ê#Î#þ# - æ ´ñJj	¼
„ºÇÒô†•'ê’¶+." ± r#%h%‘&t)•)& % ; � ¨–Ó|£¿m›ÛJgaÀ	2Gü'EN¦fçù[ðQš½ d"þ"'¥'( mÎ4VdúÂ
M*Ñ\2˜©š£b¸0—ÇÑ%" "x#ã#I$Í$w&£&Þ&¦()# « Áù0íÈŸ¯`	¥	�
o®‰7]iÒnPj  Þ ;!ÿ!7"#¤$ &Í&Ü'ñ'1(" ( Ÿ 6d ^†ô£×ó/y­Ñ^šó°Lè¢­,VâFçfO%Ó%Ù%' 1 ¹mØ4›	µ	´
§þduÏß �•Ug…`lRÈÆ �#¿#Ü#t${%€'é'&( ZÓ	
CQÃjˆ?7ž±ó ¬À z"…%É&@'”'Y)$  > ] R�Ý		«	÷	*
á
¿·›ŸXvóÄÑÿø#÷%&9&'=(e(ª(Ð(ç( ›Im1”7Rgn¾9EýÝÃî²˜Xl !½!,$%2&p&ª'ü'' ã 3�#OÕÛað%1Bšk‚5ú:Cq´û~CPm¬¯ I!G$¡$æ%a&/'¢'ö' Å ‹™!·ÓÙ#~˜
Z
ñ§oØ#Ú#^'Œ'Î(  Þ é&­Q…õãïÍsG·�– O!‘!P$w$Z&d&G'M'ˆ'Ü(ö(* c £ ´ ¼ Ç 8ÃéŠµr¹å%oš	±	D
w
¥›Ès‚ŠÖc–9€Ü< ê$(%Š&c'x'»() „ JY‘Ðñ‡˜Ÿâë*‡ê_l�ÄÑyÜAæUswýØ j"-#•#¸#æ$`'à'ã'î'A)h) Ì §!9|Ÿ	_#b
ëdtòûº"‡#$–$k'Ž'˜'»' n¸-€åuä	nS­”ß 
 ‚Û!\‘W%é%)&))! z ^ÁŠ�Ë¸)	Õ
g¬ùXÄæGj‹�Æ ¨ !!)"K"4%¬%,)/ ÖAßxïGÐ	Þ	ª
|úÃÆDJ=Q2;ä†kÎ ]œ©c€ § !˜#Ë#ÿ#š$l%Ì%‡&o'æ'(±(ý(( þ ”¡Åeö¼\

„å�G—:½ÂwÕžÞ Ißø¤Ï!Á"A#T#¢#Ï#$$a$®&ã&ì&* uÃýôáÈLçÔo)ƒÕaž©êÃ‰"™‚ÿKÀ¼†ž "ö"L#®#($Ó$Ý$~%ü%¿&* ” ç ívòüKŠk	Ü6H«o;w}›–(©ëá,¹$ßœS˜ ¤"]#R$\&:')(L(™), . w ¨ µ}÷½
#…»W@Ó‡™œÀht‰¡éõIòÍ6:Ü•Ÿ!ü!D"s#M$i%(‰)–) À)9
·
uàL‡ž�!·û=//ù,9}#%’&Ò'Œ))   ÑpsÃÌÑ
8šÈ·ÿ,Ž”ÍîÙàß“zMr´ù¬¹L# ² %!Ä!0#g#…$?%D)u)1 � ©ñ(�{ÀÓn�°×Ý(Kv	2
ñ3M¡ dT5í©¿oíüVRÃ¢ A!e"ˆ"µ"î#.%'R(ã(/ E à å÷h}À„¤œ¤ÜSbhob~
À
«¸äû˜ò(—gè�zÜÏL`iŽ˜!ÿ"¦'r(% & < � —Ô@O™ºí'	¯	ø!åH€øO•äñŽÄ›Ö¾ y%n&Š'¹'ì';( V{¢¯p	Á	„
4ýF´9Œ§ôú\y–B"ª"8'9(% ¯ $ë�Bn
†
Ã
+=NX!ø3HŽ?WØý1o}êW Q"^"á%ñ%&ß&À() n[W‡‘‘êg›c¹îF!&"¡"¬"J$§( `	¨°PÈ	x+ì]µN�Ëç1GG"ä#Î$x&ó&$  eØÏ5±eû˜
ÔîÏ"ÒÑÚo™ª¤°˜ÈÒ[ ˆ y#7$Ï%&¤&Ò&I'O'+ V ‹°ˆ	Q
’âpj½$ÍjHÏ0_}˜^¾Ð{ˆœlŒ"(Bß Œ!"#y$‹$A&f&³&Þ(ø(8 ¬ ù ÂúXˆî?É„ÔA		¦	f
§
5Gx”¢uŠÝ³ÿu±/¶¹qåcu²ötöŸ¢iÄDQ!#ô#^$¥$!&ù&Ý'Ë(* ¯©€˜ž×Ã	 
i
�
B8ä•PnÝi„‘½¢ÑõIolT» “!Ù"¨#
$Í'ò'2(2  ~1ý” « a	r	„	&6^¯	±,^0†>ÿ%C‹!NÁÓü¨…Æ3QjUQkÉ<!€! "8"�$Î&Ú&6 7e¡í,BJ_Ù$Œõ ¤ô0zŽ7	
ü
/=I_› 3ÈúØý5a=£Z®ÀeÒ±]Ó3R»> ð!'!)o)@ h   ¥ Ž¬Åþ/NZjtƒ‡çÌú¾œ	¢	N
{
“
r�³ÙT®ÊAPZŒ±üãaé.œùJn¶þóWÌðt´ÀÿÙ<a!o#Œ&2 ) e s ò i�DØ°é1Ú‹�:W½ö€L�7Yå·=´GŒg`"—"½"ê"##‹#ç#Ä$8%K%Ô%&/ ŸÇø³æ=@	F	O	ˆ
Å
�¨ÒÁôM&Jë-Ec‡ã2§]±èô 5!±!S"š"d$P%¿%Ú%�( (L)- 2 @	º­·ë	F
î
¨Ye§!�Ã#àö—$6L‹).ÇËÃ 0"‘#Ý#$&-&»&'�'Â(( ± ²ãÍë»Ùkï­¶	º
P/Ðà�„
_„®%mg„| ’"Î"À#D'e'‘'-(Ø( Ÿ5Ûó	³	™µ9VShq†= Euô"R#u$|%á&$(, › cntœ'qÕµ
ÿvâ;–h»Þ‰Ía‚ùSŒJÚ÷É®ìY !e#ì#5$&Ð'ê''(7(2 PR_aŒ:<üGÿ
y
Ê
ú
-;GR?u¿ÁÇÄËÐ‚²:A›�ª¬×ŠxzÁ õ!÷!‰%&Ö(Z)   É à]‡Ô	D7kEËøÙ.¡²È!Û!{"B$6&Ê&•'\(½(�)" \Ì;È8Ð<
Ì
Û
RV¥±8ŸCÐè§4q‚y!#ì$!%†%È%¸&A'�() Œ[s
?&ÌÆ‰@R
&oëô‘­ –!$;%±%ó%z'X(—(! ;Þâ
ˆ¸êNØ£À!¶Ûô($rÞã7 a s o!"Ä"%:&«(Ñ(è()1)1  " Z ¾ >˜Ë<,	¬	+
òwàÉÏ&’YvÜ¥&S£O<; L ù!!#p$Ê$‹%ã%×&>(È(_)3 &Dàª*DSZ	=	\	|	
p
æ¥BÌ4w�“”ºÀ¢½ÒØG½	5f#*q�ñ „"Ë"ù#m$*%&H&‚&\)7 ? ^ ~ ¶ Ó ;TI‘3	e		ø	ê
cÀ+œ¨ {Î�…þ±ä8cÅe˜±¶i†ŸÀì	 „!1$ï$$%ø%N&'É'f(¡) † ó2;Gu�Ö	¾™.e!Ê!Ý!}"«'ý' ö p)[±ÏâJenío¿=ÞèïlÝÛA»D®"«#D$ú$›'   M…œá•V8Shì:‰Æª{˜Ðêí†+-$%%3& 7 × ™,ùFþîÄ³zâäY·m¾!l"í"#™%q& n Ï *Lu„M›r2Àu>"�#ÿ$º%&0'×'^(ì(j)# ä 4Ž“PÖÜä
bñ&Y08üíQn­´ã J!µ#º#È#Ò#á#¢$|&£'÷'’) Á$_«·2Cl;ÂDðaÄÚ ” ý#è$%ç%b& , lš`_	ƒ6ûDóµï™a·ÆÐ° $"c"Ÿ"H$Ì&ð'¥(s)  Ÿ¸]�¢
¦Þ]òWfu„�°áþy"Û#N%Ø%8&©(Ï( Œš "ÒÔÚ“"$f—™4npú
BH!×#Ù#õ'# ƒ Æ Ë I7‰´ä,ŒÃÐ^x“Õ•üÿ8ú ; ¹"$å$_'b'w'�'—'â'@) ‰ €[
{C9`~¨Í£¨»… � Î!"("J"À"—#$™$Ò$Ë%n'å'(" v ¿‰ö˜¿ˆš èH“�Ø¨Ì5L«¸8ž— £"/#\#L$Q$[&C)  D ß ö'z¡®'Ran˜¹ì~äð¾óìyÎK‡"©"-%x%‰'  ê_†¯Î !Gé0ð%&ò&H'N'¿(# Óÿ	e
F“t+²/t…BOmÜh�±À/siºP!‹!’!Ø"ó#]$x$e&²&Ý(÷(( d ¤ ÄAI#MYsæC²å<›	¡	ŽœÉ‹ü`\Ò¦;= ¼"é"Š#æ#J%¾%‹&�( )n) ?ê¶º&pÔ²	E
X¦ƒgõ5‹Š/"‘"ó"Q#º&C'd'�'$  È ^9:‹þ7x
Ú
%UtQ¤�Ê÷%ê-©3‰��#ë$ %ˆ%°%y'W(¼(�)) ! µ ½ :ß©Ê);Yb‡§œ„’¡×Fd—µ)…:KÝâ6 ð  #0$î$%#%)%G&M& 6 … õ „ZÎdòìtëÅ©á@Ïk"˜%š'" Î )Kt’À™ª¶Lò7˜Á¿ìtÙ €#´#¹#Ç#ü#ç$%{&ï'¤(i)+  ‚ Ñˆã+ˆŽ¡–zÝBV_etƒûþ	7A§ùý„œ "I"–#˜$Ñ$Ê%×%a'm'v'á'ä' C  `mÒ. ç½xªÍJŠ!×".#ò#\$w%ï%±&ñ&B)( 9¨":ýXä6;Ó 	a$TŠ¦f›Pƒ£€ÉE¥(�:J5 »"##P#‰#%I%�' Í ƒc
K~•¨-6©À¾ëÎ6øü³#Æ#—$Ð$z&ð&l'™'4 ‚VW}ã:Ò`c‰TU�Ž¬¨ìéê&'Íuvwxóôõö÷789Iù ú ####W#ˆ#²#Å#	&ï&W'X'~)$ bŽªRždpV�§­çíyƒ‰ÿeysóû Y!ž!·!X# $
&õ& 'Y'¼'(()" o¹æ	>
�´¨3·Èò:‘`„@Š]m ¦!Ö!ä!Ü"!$% %˜&Å&(();)* G¤.��v¤
±
Î
Ý
ŸöáÊ‡)PzÔ
è’ðfz"¥#%$T%X%æ&ö&Z') FcåoqïçŠÿ®³®®ì•î!2ƒÜŸ!¸!Ž"Y#ê%&*&•&'/ =«SÊ	 
�
ezòT�J	îWØe@®=ƒìÛzŠà W[{Ó"t’ôG M ü Z!{!c%S'±'½'€)$ Úpý*	Ö
õ
ÖÈÔ!	¸H»Ç!�ËíAšú f n §!×!Ý"¡%™& (™(-) Û Îçù‚'
hýY=µC4‘Á2"!Æ& { ‹]¥ò
­(úB©ók¦½^ j l!á!*""$?$5%C%F%­%Ô&(' _ºÂÌ¹	"	ï	?
§Åç~‘¾úÉ;Œ’Ø.UaÖ˜…©‹Q ] å!L"%(	)<)- zˆE×¥�´_
“¿Ô>qˆ*Õ¢(q‘ÅÓ!� ¨ .!Ò!	"k# $$›$Y%³%ç&p'{(…(²(þ()) 0HBjàwð,Hß	ä	
«
Þ
ûÇEXÏ–åQ€lH{“�Š )!D#™#Ì#m%&W&¨&['' 7/É	Ñ	u
²
AÄ÷?ŽEÓ‡Ïé¦ñöšªdj»!Ž#&$U%Ñ%õ%'&…&ˆ&K'~'ç'1 Ñy‚
Æ	l
¥
Ï
}ì Kù4>Râ-3<“Ë{š² &^g{ŒÔèU † !Â!{#¦#‰$=%Í%÷&(P(‡)4 ™ ð ÿ ZgŽÆ×-h=pðEP×—è$Ü¾ ¯´¯ñ¯r"3p!;J[¥²¯Â"É"c#ê#$b$&+&'5(Z( —5d½ær)˜,›¯íâ„µZ#Ð#$ã$Õ'% 
   ¢G
…ðH‹¡xê¬–yßù¥E -"�"ñ"U#£#í%K&–&¯&ä&í&Q'9)' ì î •ÊÌf÷]
Næ‘;ÃÅzÖ–ï‚Ÿ79YàÝt! !¹!Ð!B#$ë%¦&|'(& g!
æ
ËÕóèZ©ÖÉN%TŠAƒø{á|„ÊÔß‡H !|!˜!"÷"Ô$^%¨%ý%²'* éâTÉÚž
èf{ÑUp}Î|„¯ªë|Ä©ÍL“#“õŸ ý é!4")$‘$Ú$Þ$À&) Ä>õ³¬	š
MÝ‚*Kïb€ÇÙ#¯`„í¥ñô vü/XuN v!M#¯#Ç$�%¥%T') vþ›Ë	(
»Ä‹ŸñX	\fš@>ÃÜ‹‘<\¡¯Á½[!h!¢!9$d%%¾'D(�)+ • è PL+	l	#
S
Í×Š-l¬âE*+—J-–¼ß}î(û( ¨!^#S$ò$]&š&i(‰(“(š(3) "óþr¡7\~<n"DÃÒ…ÙýÇ Q›¥Ž!Þ"¯$;'…'G(b(& qwýà~‹×
ÉÕËòö%èî:`2 g | ‘ ™ Ø!!";"�"#`%•%¢%!(*(M(Ó(.)I Ûîÿ˜Š	ö
ÝºIPSVp¦©ßòx~œ±
VvÑù)Aª¹Èìï=IaâåºÉÈà"An‘Ò�ÌBaltT o u ¥"ù"�$<&R&­(š)7 ø=¤”»¼þXõÔÝìˆ�¬&Zi¢«·>õ[¶Dó5Î7j`Û’ž1Ö3–	 x !t"}$¸$s%C&( © ¦¸·ø‹
†·<8!ý!t#Ÿ&Â'O) / a x Ü b¶èƒ(
iAÁ¥Â²€$N$j%ª%Ç&*)f)& Ï~®ú¾
$�šu{ŠÏØêöJðLß8;eÂÝ�-#!?!~!"E"?&6'Š)—).  X | ø…é	:
¸
è
®ä)9‰¿ÅyáC‚,ž"Csš¸ôHl´ü~¾05ã m!¯!@$6%Õ& Á^vMlJß_9 á ÿ%(ê( Œ¦ˆýª»^#àk î ÷ W!â!"~##$%G%®%“&þ&(�)" XC*²ä
ó
ûÒ#*Ÿ§>}¹Ž”»$0ú-:€£+"=$D%U&Ó'(y(ƒ(/  è`¹+hjÝð÷-¯µ)ÎûAÝF�Ù6\sµ·×æ'I|ÚúD&_~$ & ì 1#{$h&´'((�(+ ¹ Òê»�c¼Ãtð	Ò
×ê¨l•á’¿]ÿŒÎN)KiFXM³ 3#¼$%Ã%"'¶'à(ú()( qÍÍ›É¸ù�ÆY€Ú2Ÿ­º†º‰åðB J R ^ c &!q!‡!´!Å!æ!"Æ"#h#†$@%µ&=)T ¢iºÄ	#	/	�	@
9³øÚ /èP ÐÔ×æï'¸Ôá}à”Ê<C{�“À*/VYbnuŠãúf™¸Üèü*ª­†Œ«³ÀÌÖá!š!"M"Ö$%j&)
)E)v)- ªÔ{©S|ëy±.X	3
�¢Ä–;ÙeØüb?WD¤)Ä© µ Ú B!S!f"/%'q'|(ÿ()y)A ‘ À û {ÄØ¦� o‘‘ØÞLŸµ	¨	-
`
 al4NŒ!â”ŽÜ£À(–áS’FÆ"%N£ /!o"‰"*#5#“$¾$´%t&o(³(¸(ä(, ‰œF½))Ô)•¶éC	L	¢ßìUc6Õîx£ÔçÐ÷Ó!¶"Ô"l#ï#$j$à$%Å%Â&„)ž)E ò‘Peš�ÁåËI	R	U	w	 
€êòhk}µÖ³Œ»PÀ¬ªr‰Å+pŽÄÖîýWvMµrNÕ= ‚ Ï 
"V"Y"'#$œ$§$«$Z%Ý%è&û&@(S(†(H)= F Iñ¥Zi”áï¥ÎÝùÚ	à	å	ò	û	

ôzÈåy«óYw£ËsèÆShR‚�¥Ës©ZÐ”*!ë!##E#n%#&©&s(a)¤)- æCy~ÅTcipxCVv�	
¬
I¬üHºw{Maj•Y‹ Ç Ò !ü" #š#�%''$'§'!  á 1Ák…¥�ñ-ß
€üí™ì˜«ÝIÆ|Vž�Í#ö#r$Ÿ$X&\'- $ : Ž ø/¾ÍžIIci	Á
¹F)MÑÐ[”—eæéø‘ZPm-O™! 6"Ì$%g%&Ý&)( ' = \ ž šÖ		Ò	³
BÎ¬ÐTç_¬6ò+QÐò«æe¿ �#z%„%Ò%ö%'è'<( ˜0APÜÂùr±F—kœ«ÿ ¼!+$å%o&L'‹'Í($ » ð8†ä$n°	C
v
"¤@aÇ	r�êbs�Ú× '%V%(&‰&j'º'ß'í'))= Õü@ÄÞ»ÊîÇ(	Ï	©
Ô
	fùƒÂÅæøIÓP�
(1F–ÔåÁéãˆ!iŠ�Å§÷\›!'$`$Ü$3%û%†&ë&°(ü(( ì|ËÐ
7Û5?Gª¶n†+:s�Ìí~œÞ y#q³ø›Ã!û!C"|#„$>%9'(ˆ)( �Òz£&	u	®	~ ·L úcS48”ãì¨Ux�_h�•—Õ¡ !m&¸':(Q(â(  ® #œZA°Ç	m
…
*íwW~´>V×æn|éV ‡ P"]"«"Î%à%Ñ&H U WŠü >ƒ—@	q	ƒ	‡	Â	h
¦
á5A¡þ°ã.=”µ¸#Ìä Mt¼õû^|—¡§Ð]½Ï2Pz›k‹¡!'Hhk#§#	$Š$Ù&ø&Ì'Ê(K ñ hÆì«�Ø÷.i‚½6	?	M
‡
’
Ä
qèØ§0OYÙ�û2Çù4I9¼dòDb†°ïKs�X¿¶þ2<Q\³°ºó `!ï!R"_"
#c$&'Ÿ(K)/ š °  ±âžs¬òí
O.€˜´:"ºÝRp­ßˆ$�–fIÙöKëX !Í"d#ë#4$,&à&Ï'6(Á(, [ËßÇûF\Ïr
É
ù
,>FQ>Ë6°Ê	@nÏØ¦pw x!•!Ç!ô!$ò%&·&[(): %C2	<	d	{	~	
o
ñßé%AM‘3¢¿ Žµý“Û¤Ú%R°¢¼°¼#4ehq"p~ë ` r ƒ!"Ã"Ê"o$É$â%)^) Ö o˜(°Uˆçí½y-kÜdºã…¶C­"ª#ù$ 	X^	î`ÅïÃÏž"Ñ#Ö' 6\³eÏ}œ¯Ô”ºG!'"$$ä$ô'¨(?)% õ®¾’s*±„—¾‡™Ÿ’Ûgœ×�§°¿Ëò.4èëh7†"¢"¨"[#K$,%    9>Þ@HLB±�†_‘ö´,„‰2."�"ò"å#L&V(  B � (©µ ñ^d‚—Ìàsú@¦‰!"H"û#[$î%°&£(, �b8a
©Q�o,SŒO‚¢§¿Èæx‚ˆþ¤½è%Ì6Hò�!¶!O#V#±#Ï$&y&î&ô&V'(; F£É	
œ
£
õyñ€æIí³É†­¶×(OdyÇÓñ	?~�ë­ëÚ1?VZz‰‘F L ¥!Û"¤#S%Ÿ%—&å&R':) Ú ÍÙø¤ü!	ÕÓ <½×-TÀ—ÊP \ e k!B%Ó&(3 /yiÐ6		Å	
^
k
3’,DÎ¾Ò=p•¡±¥G�zÄ™‹ÓçT … ‰ (!º!Á!Ñ!C#j#z#$ˆ$Ð%&„&§&J'}'1  í ï f–ÉË4,g<ïDMOÖ–#ÛÄyš«qox8:XZ±á´D s!È"ð"ì%J&¥&P'4(8)4 è²šfÙ	™
Ê'ÐçY¨ÃÈMÆ[{Ì_Â÷¤ðóu�ƒ ®ÉÞ!g!u!¡!è!8$�$Ù$]%œ%§%C(. ‰	"
R
Ìq k[}D)ÊñIUuÐ<`ÂÈÑ
„ü@mÆÞ_|k¤�! ":"ø"Œ$®$ñ$_%Q&F(h(# ` ¥£­¶“ô%ª¶Î×ôZi~_d�0œÕ,;>!}!"·$©%B&Á' W W±ç
ã8ˆ¾ÄÑk�)º™³I|�º#Þ"â¢à "<$þ%x(O ¸ Œh²*Öé÷gÜïö´OŸÓÖå&\þ1@‹ÍBEœ¿5X[m‰å&Hh{çù )CW¬¹^}…ª²¿ËÕàˆïI ë !†!™!"#z$Õ$Â%g&´&!'³'ß(ù(< ú Ãz€êÊ”§	Ÿ
¡Ã`k•é:g|‹Þë´Õ²ûº�«¢Äu•C£MEÏ$ö Î Ù R!Ó"i$’$¦$ª$ß$
%Á&ú&G)x)ƒ)�)' Y�BUh	�	ú	HyGª¹Lv¢çÅRdv÷ªrÅ”X  ê!5"õ#f%"&Ü&£)1 ûï…ãÕ#mÆÛ
	Î	B
¨
e£Í`«ÒqÏ0S°Eèæâ«5‰Ùåþ *$_$Û$&%ƒ%ú%Þ'Ì())A ­ "‰›ÿ@–Ê	%	t	‚	†	g
)6à¶vVb~ }…·"rëì}ã›³– Î"1pwŒ÷Š Ugjšåm”  O"\"#ƒ$l&Ð&Ë'E °ÅÊªö�¼ñ1	5	;	c	z	
‘
È
ì
N-³/9Þ@L1ÆQo¡Þ‡�ü#€’Ú£Ù$a…îJem¯dµPv¹ !_!w!‚!”!3$È$Î' Õ ­°ƒ–†ÚfjÖŽ¾“ÄBÂ�"©#$ó'5 A € =€ ?K´Ÿ›

nåðc�RÈN^…ÖÒ>}�ê¦Çõw³ý0>UYˆ£¼ˆËœ!¤!"Ú"N#°#ž%U'(. .x3h™Ø 	Ä	j
;ÉCÕ&•2+½,S°^öpò�Fw�­yÃO j!Ø$¦%3(7)9 V¤¢¬µ7±)Ÿ‡fîõC(ÐóYÒ
 ýhÌ›Ç	4l%?z›Åæÿ:V^Ý!¸]±¡¾î=!""9";$P&À'E(w(4 !þ•œ“"lg	s	�	…	Í	(Â_jµÌ9_a¸ú DŒêQÃª4ˆ!BqTlÎØäWh$‚$e%‚%Ï&Û&‚)* wÿ2b		%².¯1?…¼Å Ù]Œ"�ÕR
Ž’=EŽ¢ÂÊÃ!^!i!�!£!(= ¡!kÌ	'i°
\]^-_`žìí�‚ƒ„&'òOPŒÂÔg©3†‡AkÄþ	RSŒ�°Âã
!!!\!]!""":$g$€%�%¿'  =(  ›GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                       7                                                                                                                                                                                                                                                                                                                        R                                                  	                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
                                                                                        	                                                                                                                  	         =                           =                  
                           
                  &                          :                                                   
      
   	                      &                                        	                              
         $         '   -                                 W   ;                                                	               #                                    0   $               /   O   ;      ;   7      0   >   .   (   Õ   s      1   ¯   ¦   '   .   6   "   •                    7                                                                                                                                                R                      	                                                                                                                                                                                                                     
                              	                                          	   =         =      
         
      &          :                 
  
 	        &              	          
   $   ' -           W ;                	     #            0 $     / O ;  ; 7  0 > . ( Õ s  1 ¯ ¦ ' . 6 " •    ù  £  £#  £™  ª»  ³‡  ÀO$!q(›UH` ¤ªö
	‚	B
'(Éj¶%&|•²Ò9ƒ[Ü&ÐÑYZÚ
NPQ›1BE%m£Ä"U¸ýYdà  Î à †!�"E(6$vA5ALrÁY"G9hÑŽ&µt ·?&´Ë!ë!äg­$)Í& V

q¡=
/v+ ÃT	GÉ(ªÚ<çŽY²ÃÄß÷Å	B	H	K	T	]	è	¸Ë®ØOXiëñø!.žÂàwÏçxïCsBÆÏÞç Z¶äCgpÔGP4®²jv‡M¸ä8 { í )#­$É(û())))àGñìÃ6x�ô4tÌž¼Â6$€$Àt6Ìž¼Þ6$tÌž¼6$¼¼¼¼¼AÖ®		5AL13%Ò%9'aaµÙ ý�¨q#¼&Â	zi 
vzB
Íª�Á°…5	ÃC:]õ�ñ"…]‡	¡W	Þ½«•½«<C sn‘˜…I¬¨þ
Å[?ü/0
(ª¼‘
îè:ý H´š¨%¨üH# v¡#â&ÂÅBí{D(í¬¨þ
[ü	A[?(<.ø+Ñ'á<(Zƒ%) 
‘¢W	¸& >-	‹	?®ÊÌÒK{º\»ádâ6a¸¾$)ˆö“7y ‚'†%‘&˜Ý¨ÒIÁô±=-E‡Ò§ ¿%š)lsz 	}bZâO.€­Í"£†(Óå&œ  (Â-	‹	þÒß{<ád’.ø+S&Ñ')(s  9
¾ ‡8
¶
ßú
Æ¡Ëqž¿;Uø!�&Ö&Ç(;ø!Ö&Ç(¢ÕC(<óYh@0Ø æ$ìxSØ>­5@ÉÍJ[0ã7;Ôžø!Ö&Ç(¨Ò‡œ
Æ¡Jf"6Y@¥º0½wÇU�g†(k!Ó& -	‹	Ò{ád)0
é[ Ëµ�!†'9ëø31“Îh$‚)ßeßë%ØÇ!†",% -	‹	WÒ{ád¶@ ²!)ü ãÜG ¬¨©>-	‹	£	þ
?\¯[®Wã@¦Êüý#œÌKbÒK{º\/01»ÄÊá;dv�â6a¸h¾Ü !$e$Ž$a%¯')«½’
ïè�$>‰."(!C#§&x Âª%*)ê 52Eò~$¿œ zY?K£Õ5	ÂÃÌ*9«.žÏvˆ¾úÜï%1CDÅÆ '(Z^æi™«³"BCår£ÄÞšâ^På¹^!x)Põ	€pZÉÐƒŒ!"^%q$» b'%ˆ	ðtÏ;_lj‹$çiÙ®±ž-L /	ð±=ÒÃcî!—+u‘Û¤Rx ±Â=Òg "ª%?&*)J”€(À6
PI(Õ«Ïæ«å_Ø
SÆ¡Ø>Jf"6Y­Ë5@¥ÉÍºJq[ž0½w¿ã7;ÇÔUžø!�&Ö&Ç(	²
Ž#~'ç'¾ Y?KÕ«Ïv^æ«rå«xD P'8)s·	n[ 8Ýç¤“ì0	:	>	y	œ¦Ôµaeixô*x¼Ñt�ûÿÝ;ËØx$CyÙåY®·
cjƒí
 w Í ò "#©$ž(2)F)J)Õ*«Ïˆ¾Üïæ™«³"åšâ^å< [ ñ¾ ;(¿ü sßôã�œ»Á—ÓœÜG¾?#J# #¬#¬&½&aÊåŠ§›(B	K	Ä%#Am
n|éà%C(*Üïå^6ë³#Æ#z&lBžgN%A„ÊÔß!˜!Ô$
Æ¡U¦â«½’
O.€­ïÍ"£è†(Ó�$å&Pš�I	R	U	hV"'#C<	{	o
3“¼eÊ" 	Í(6_¦Š°�áî<†¬z
¹
Í
S(|{à/Dƒøµ;ø!à#Ö&Ç(Ì_-h÷æ	²
Mp×>Z;XC€Øƒñr#ú§!-"�"ñ"D#Ž#‘$~'ç'(‡)Á  Ö|!4"X™t;j*}ÖŒÜïªÅå¹N^Õ "#´&†(H)µ/Ô¶$ÙÊ<ß%}ÖŒªÕ H)(><s‰(!."C#§&]'W‘@&f ¡%]–·ê|E.&½(âò =]GÔ%æ
ôœ»Óœ?# #¬&tà‚�Â˜�¿õ"K#­#}%¾&GêºvÝ
ƒP
’‘"C'Z'd'�'zÅªêö�¼5	‘
¡Ã•:/û1ÆCa…£îJµP¹Ù R!_!x)ë •�6{'(ÔÚÀ/
6
•
2¨EòP~$I(Od™œäH	Q	T	}	j‹O�2�ÃQ¡L´»»gÔ< _ q "U"X"&#n$Ü%?()])­©³8	O
ÿ
�Æ\¯ÛèÛzdäó@Aoý#KY01ÄF†ô#o@CRUe$´$Å$£%(4)"
R
ÌŸkCD()IÇ	?ÅÞ^|9"ñ$P&E(h(Ù]÷&'"(+(‚¥
 â-<“{š &¦#‰$÷&­©O
ÿ
�\¯ÛÛzoý#Ä†ô@RÅ$£%Od™œäH	Q	T	j‹O�ÃL´Ô<U"X"&#Ü%?(}	�2Q¡»»g _ q "n$)])ûÆÎ	¨
eÒ0èâ‰_$Û$ú%5c…¯K¡UíÜHow}›(©ëá¹ßœS¤"™)Ã€(¹²H¹· þ—Od™œäH	Q	T	}	j¹öORU‹¥¨ÛÞñO�2°³žÕäø@[Çî?�ÃäDQWZ¡äGLg´Ñ»«»`gs©Ô< _ q t ê …!"U"X"&#n$Á%Ü%;& '?(¬()])zÅªêö�¼5	"
R
‘
¡ÃÌ•:/Ÿkû1CDÆ()IÇ	C?a…£ÅÞîJ^|µP¹Ù R!_!9"ñ$P&E(h(x)?ÃÝÓ
‚Á	'À  h[!2%ê&¯(û(Ü	ª	)
¾¶ž&d(æ(ë†
Ã
NXø3HŽWý1Q"^"&”ÞëÏöÓ"i$ß$
%Á&ƒ)�)„s��c(W)üÇæÓ
(Ôéˆ!'$Ü$û%y^
’¾=p¡�ÄÑ!j#$ ¬í
"ß–K,&Á(¬î½�¨¥Mm	þ
Ñü~)úºŠð?;V (vÓü(Š°+â¡=T½5íj˜${Œ"?˜ ß µ"î#ñ%)(L() y¬^
í
"’¾ß=p–¡�àKÄÑ!j#$,&Ö'Á(‰œ))C	L	¢ß6ÕÔl#$j$Å%ž)® #œAm
…
*W>V×n|éV P"]"à%ÐorÂ™þßK$!f#ª)Bã
"¸“T&‚(±)fîõÒ
 ýÌ›4l%zæÿV¸]±¾îO‰,á*•'' ˆ(’(2)d×·òv¿ãì	—
ï
ÓöRm¬¸ÐÙ÷n—¯ Z ¥ 1"6$Ïúó
ûšu{Jðß8;}¹ÝŽ»-0#!=$D%Š)—)™ ÿ z×¥�´ý_
—ó“©ÖÜ!‘šJÅ!.!|!Ý"c#ê#¨%³%+&5(²( ›

åÈ…ÖÒ>}�ê0>UYˆ¤!Ú"ž%‰ +Ú¸ÇQ‚c¸!Y=°"±$'$)ç	X§ÂÇLZËÁ£t!f!ç!�$\%›%B(	¬4Ln/b�NvTk(+ bk_ëáø’¼Ìø™%­¶I#"b"Ö"ñ#<%v%&Ë&O(r)†)s¼ÁÄà·	0Ž`nH#¢
ðHxê¬yE £#–&ä&Q'9)ì	ï
ö¬¸÷—1"d×—
ÓÐÙn¯Z 6$bëáø’Ìø™%­<%&O(†)·òv¿ãRm ¥ · þ—¹öORU¥¨ÛÞñ°³žÕäø@[Çî?äDWZäGgÑ«`s©t ê …!Á%;& '¬(® #‰œœ)A)C	L	m
…
*¢ßW6Õ>V×nÔ|éV P"]"l#$j$Å%à%ž)Øµ	Ïßl ¿#.±
öèð%$T%ò6;:'æ
áý% è`¹+hjÝð÷-¯µ)ÎûAÝF�Ù6\sµ·×æ'I|ÚúD&_~$ & ì 1#{$h&´'((�(¸ Œh²*Öé÷gÜïö´OŸÓÖå&\þ1@‹ÍBEœ¿5X[m‰å&Hh{çù )CW¬¹^}…ª²¿ËÕàˆïI ë !†!™!"#z$Õ$Â%g&´&!'³'ß(ù(þ!O—ß }‰,¹ORU¥¨Þáñm°*ø@CÇîäõ•Øçí9ÑŸ'P`s' 1 t { � €"#”%;&„'a(ˆ(’(¬(Ò(2)u y^&—¸ëˆþ	d
E}ANlF
rK¹/&¾(Ø 7Ç®ó`6æ§¢ë¡
‹ó~×�¼Á1{«±;È7&,ÅïPb sôÖV‚>¸nÍ ƒ e!¿!í!î"÷$[%š%A(T(I)¦)¡çéb».	Œ	¸ikÙø.®à(ÏX·Ó|Üž)t¶â(Je·ÙÛûE%ä% A b p!³!"Å"2#»$%i&µ'(€())Ö o˜(°Uˆçí½y-kÜdºã…¶C­"ª#ù$y ý t] àõ·Ý	«ä:‚W�¼ÃÂFv…˜Ô��ÝH¿õ"@#K#S#¡#­#$k%}%«%­&¾&â&+)xÈ™~³9	Æ
ŸÇÞ©éEÓJÂõdåóA²ãLúZ:>1G#.F\oˆÓCbu¨@U Ì × é ›"e$µ$À%(5)›)‰	"
R
Ìq k[}D)ÊñIUuÐ<`ÂÈÑ
„ü@mÆÞ_|k¤�! ":"ø"Œ$®$ñ$_%Q&F(h(' = \ ž šÖ		Ò	³
BÎ¬ÐTç_¬6ò+QÐò«æe¿ �#z%„%Ò%ö%'è'<(Ø ,7ÇÜ&x®ÈÝó`™6f~±æù§@³¢ëï	9	x	K
¡
Æ
÷
‹ŸÇÞKgÊÓó#7Zl~“©»éE­¶Ó×JÂõWd�¼åêó AQ�¦´Á1{²K«±»ã;LdswÈú7ZŠ™¥«Éð&,:>bÅ×æïþ1PbÊG ¾#.BF\oxˆÓ\äsÈÍôCbmu¨ÖãNV^w‚>²¸é4@Un¢·Í  p ƒ Ì × é !e!¿!í!ò!"Z"›"¦"Ñ"î"Y$e$Ž$¨$¬$µ$÷$Q%[%š%À%(A(T(u(�(¡()5)I)›)¦) ' = \ ž Œ˜šŸ "¸ÒÔÚ]“"$0fAP�š¢Ö—™Ü		Ò	
³
B¦ÎÞ¬Â4Ðù]ròT±FWfu„—npç_¬6ò+Q�°Ðáúò
B«æþekœ«¿ ÿ H!¼!y"�#×#Ù#Û#+$N%z%„%Ò%Ø%å%ö%8&o&L''‹'è'õ'<(©(Í(Ï(À)9
·
uàL‡ž�!·û=//ù,9}#%’&Ò'Œ) " Z ¾ >˜Ë<,	¬	+
òwàÉÏ&’YvÜ¥&S£O<; L ù!!#p$Ê$‹%ã%×&>(È(_)ú °ÃzÅÊª€êö�Ê”¼ñ1	5	;	c	z	‰	§	
"
R
‘
Ÿ
È
ì
N¡ÃÌ-`kq•é:g|‹³Þë/9 ´ÕÞk²û@L[}º1DÆ)Qo�¡«Ê¢ÞñIUu‡�Ðü#€’ÄÚ<`£Ù$u•ÂÈÑ
C„ü@am…£ÆÞîJ_em|¯MdkµEPÏ$v¤¹ö  Î Ù !R!_!w!‚!�!”! ":"Ó"ø"3$i$Œ$’$¦$ª$®$È$ß$ñ$
%_%Q&Á&ú&Î'F(h(G)x)ƒ)�)ñ hªÔ{©Æì«S|�Øë÷.i‚y±.½6	?	X	‰	"
3
M
R
‡
’
Ä
q�¢ÄÌèq–Ø;§Ù0OYe ÙkØü[b}�û2DÇù)Ê4Iñ?IUuÐ9W<`¼dÂÈÑ
D„òü@Dbm†¤°ÆÞïK_s�)X|¿Äk¶þ2<Q\³°¤º© µ Ú ó B!S!`!�!ï! ":"R"_"f"ø"
#c$Œ$®$ñ$/%_%&Q&''q'F(h(|(Ÿ(ÿ()K)y)³ Xm�ÂÏè†¦—ž¸áê)^kt{	m¬~Þ	cñ[�Û·#é$è%.x3h™Ø 	Ä	j
;ÉCÕ&•2+½,S°^öpò�Fw�­yÃO j!Ø$¦%3(7)ôù$p®±»®¸>¥‘	I
Z½ÿCíž¡'>[j£-\·áH8kLÜã Î4,
/2>. 1!u"$V$	(Î )Kt’À™ª¶Lò7˜Á¿ìtÙ €#´#¹#Ç#ü#ç$%{&ï'¤(i)p Ø 7¯Ç®ó`©6æ€§H˜ÉžÀ)’¢×ëY	Ã	 

9
i
�
¡
·
u‹£Bó<~8×yäý�•¼\Áà1LPn{#‡«±Ý/;@ižÈ7„‘�½!&,T¢·ÅÑïPbû= Íõ/5Io/lsôù,9T»ÖV‚>¸nÍ  ƒ 2!e!“!¿!Ë!Þ!í!v"Ù"î"}#¨#
$÷$%[%š%’&Í'Ò'ò'2(A(T(I)Œ)¦)Û Îçù‚'
hýY=µC4‘Á2"!Æ&  9ˆ     l   …         …s"K¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆu•À»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒü]øTÅ@WÑ;»Ð{ŽÌÜ5Äx!'=UªLäPDF1-&”ÀZFÌ`V°¥@EU™ZUÙ’`54ä\k4¥¾ŠEvÑC‰Yà9×ÛúÑÐm^‘¶™–t“_îhˆÛT•G}AN¤uQ5]T”}ótÔÃÞ­�˜@u E!i™Y•$•ZÚA$ßßPÕD‚4DUQ4€Yê+è
èÓAMæ4ûXs‡	Ðôu¦‹‚d�4Ñu¨ášÍtÛŠ5íÒàµ4ïCQoƒˆùPmtï€€(î^Ú�÷Ž#è9(Y[¸F�mÜ~€tEiu§u:YÞúZ[5_MEQauš¤e`5* Z
—eÕ â	™VšÙa’A‚B¥J¨âz¹’D¤4k}¦Ê´~ýÄüt?EP�ÑÚÆ’ìp›
^©TU €t� TY$vY5ÑÙÕåE§UuiÕZÕj›äšcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'*fSDì©!|´løÏosŠ¡ûÙk‘9>Åì²§[=þÄŸÙÇ'á¦5é¾˜b~c“›é†/Ñç6@oã½31A¸ÆÎð�ËH7lýÒÿJ ÃZ>DPƒ0	^R²'ƒ¼ú«ñ |™”�$³È\®ªÍp*<ô]Ó…:Ä³nï­ÌüóùøCÏosÂ„³+�>üø‚>ø^2óË�”©S´,GÀú‹Ã;úùùÓÃ»Üê>ã£µîrê*Ê®+Œò�ÔâùÎG=öLq�Ï`FÄ�6ßþ�+‚õ46]FMõC?‡ëOùøá=È`9Ã®}ëùÿL9Ä_úøÜö×\0ç}wólE'pÃ#�ÈN´çÎq2zÔ €ß¿Ä2­ñÔm¼Ó\÷È~7ý€Æ\Ž:ÏÍúÓã1ÃyÓ¬‚Ov7­DÏoÓo� ?Ø¬úóÌ�ãt×|Nl�'ÓsëQÿÛ>³Ñ¿3´²õ®<�Ã|ð÷Á»1DºåØÂ�[ðþañ'S÷Ï³ßÌqËýÉÍþÏ<ÊÏÏs=ß,oÏvÄÀ·ÄãQ{¯·3,Çl5€³3Ì Âà}ÏL±L Â+<FÃó6„Â¿3Mt`PûàÏ7ÃõüÈ+à÷Ë»?}P.LòÈïðHÃÄÌðüúøûÌÿ5ûþ¹ÄÃ°ë|·Ï±üP|Íø#aüÔü#á[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢mŽhëò`êÚ®ûè™[à£‹º˜A(ƒò‹C\ƒ ˆÞ"£‡5ÿÞ
 Ú=÷âÓ¾¯êÎ¯þþh÷¶Ï>>�Î8 ì8 7‡Zò¢ÀáËêö;>"�~¸Ì/®9ËúÃû[ÓëëóŽ<;Œ>ëÌ»Ï¬þÑÄ°nþ óïóàŸ©GÖ�CN$n“ÞªU@àP9k{õÅYFãªŽ‰à.î
GÑ¸O€Nî^@_Þ:SíeN�¡#­I"#òâˆ‡¸%äFþÑþ©-"[¹4’JØ¶é¦´š™z5EafÑè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.äæüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóì>á9¯E$„ê¨ù¨Ñ?V²[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=øråöšm‡i~eVC&ÜCú²ìÌ«aÂ~áÝ�;ðGtè¸”Á�4u?-2Ó(0"À8:^c´M'„Hßf0ˆV|ÝÉAgÍ´3ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ=N            •  —  ñ  	   	  	v  	{  	Š  	‘  	“   „  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  -Z<?xml version="1.0"?><Features nextValidID="24"><Feature recentID="23" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2422" maxFusedRunOn="2422" detectionMode="exactProteinMatch"><Segment range="2333-2359" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2422" maxFusedRunOn="2422" detectionMode="exactProteinMatch"><Segment range="2363-2389" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="21" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2422" maxFusedRunOn="2422" detectionMode="exactProteinMatch"><Segment range="2393-2419" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4891" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4508" maxFusedRunOn="4508" visible="0" detectionMode="exactProteinMatch"><Segment range="4892-4903" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="5724-5740" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="5793-5814" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="5748-5754" color="#ffffff" type="standard"/><Segment range="5755-5772" color="#ffffff" type="standard"/><Segment name="-35" range="5773-5778" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3817" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="4420" maxFusedRunOn="4420" detectionMode="exactProteinMatch"><Segment range="3767-3820" color="#cc99b2" type="standard" translated="1"/><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7722-7826" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1804-1921" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5387-5508" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="458-583" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5527-5662" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-414" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2011-2214" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2484-2695" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5082-5315" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1076-1309" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2708-2976" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4421-5008" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6102-6690" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3821-4420" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="4420" maxFusedRunOn="4420"><Segment range="3035-3697" color="#05fd14" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7652" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6861-7652" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7653-7721" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>2494204a-9068-4b53-a17b-c8d42d450db0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.6.7</Created>
<LastModified UTC="8:32:28">2023.7.13</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..8324" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..8324" colors="black"/></BottomStrand></StrandColors>  :     t   …         …s"K¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆu•À»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒü]øTÅ@WÑ;»Ð{ŽÌÜ5Äx!'=UªLäPDF1-&”ÀZFÌ`V°¥@EU™ZUÙ’`54ä\k4¥¾ŠEvÑC‰Yà9×ÛúÑÐm^‘¶™–t“_îhˆÛT•G}AN¤uQ5]T”}ótÔÃÞ­�˜@u E!i™Y•$•ZÚA$ßßPÕD‚4DUQ4€Yê+è
èÓAMæ4ûXs‡	Ðôu¦‹‚d�4Ñu¨ášÍtÛŠ5íÒàµ4ïCQoƒˆùPmtï€€(î^Ú�÷Ž#è9(Y[¸F�mÜ~€tEiu§u:YÞúZ[5_MEQauš¤e`5* Z
—eÕ â	™VšÙa’A‚B¥J¨âz¹’D¤4k}¦Ê´~ýÄüt?EP�ÑÚÆ’ìp›
^©TU €t� TY$vY5ÑÙÕåE§UuiÕZÕj›äšcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'*fSDì©!|´løÏosŠ¡ûÙk‘9>Åì²¤[=þÄŸÙÇ'á¦5é¾˜b~c“›é†/Ñç6@oã½31A¸ÆÎð�ËH7lýÒÿJ ÃZ>DPƒ0	^R²'ƒ¼ú«ñ |™”�$³È\®ªÍp*<ô]Ó…:Ä³nï­ÌüóùøCÏosÂ„³+�>üø‚>ø^2óË�”©S´,GÀú‹Ã;úùùÓÃ»Üê>ã£µîrê*Ê®+Œò�ÔâùÎG=öLq�Ï`FÄ�6ßþ�+‚õ46]FMõC?‡ëOùøá=È`9Ã®}ëùÿL9Ä_úøÜö×\0ç}wólE'pÃ#�ÈN´çÎq2zÔ €ß¿Ä2­ñÔm¼Ó\÷È~7ý€Æ\Ž:ÏÍúÓã1ÃyÓ¬‚Ov7­DÏoÓo� ?Ø¬úóÌ�ãt×|Nl�'ÓsëQÿÛ>³Ñ¿3´²õ®<�Ã|ð÷Á»1DºåØÂ�[ðþañ'S÷Ï³ßÌqËýÉÍþÏ<ÊÏÏs=ß,oÏvÄÀ·ÄãQ{¯·3,Çl5€³3Ì Âà}ÏL±L Â+<FÃó6„Â¿3Mt`PûàÏ7ÃõüÈ+à÷Ë»?}P.LòÈïðHÃÄÌðüúøûÌÿ5ûþ¹ÄÃ°ë|·Ï±üP|Íø#aüÔü#á[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢mŽhëò`êÚ®ûè™[à£‹º˜A(ƒò‹C\ƒ ˆÞ"£‡5ÿÞ
 Ú=÷âÓ¾¯êÎ¯þþh÷¶Ï>>�Î8 ì8 7‡Zò¢ÀáËêö;>"�~¸Ì/®9ËúÃû[ÓëëóŽ<;Œ>ëÌ»Ï¬þÑÄ°nþ óïóàŸ©GÖ�CN$n“ÞªU@àP9k{õÅYFãªŽ‰à.î
GÑ¸O€Nî^@_Þ:SíeN�¡#­I"#òâˆ‡¸%äFþÑþ©-"[¹4’JØ¶é¦´š™z5EafÑè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.äæüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóì>á9¯E$„ê¨ù¨Ñ?V²[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=øråöšm‡i~eVC&ÜCú²ìÌ«aÂ~áÝ�;ðGtè¸”Á�4u?-2Ó(0"À8:^c´M'„Hßf0ˆV|ÝÉAgÍ´3ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ=N            •  —  ñ  	   	
  	  	  	v  	{  	Š  	‘  	“   „  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  -n<?xml version="1.0"?><Features recycledIDs="0,1" nextValidID="26"><Feature recentID="25" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2422" maxFusedRunOn="2422" detectionMode="exactProteinMatch"><Segment range="2333-2359" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2422" maxFusedRunOn="2422" detectionMode="exactProteinMatch"><Segment range="2363-2389" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2422" maxFusedRunOn="2422" detectionMode="exactProteinMatch"><Segment range="2393-2419" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4891" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4508" maxFusedRunOn="4508" visible="0" detectionMode="exactProteinMatch"><Segment range="4892-4903" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="5724-5740" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="5793-5814" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="5748-5754" color="#ffffff" type="standard"/><Segment range="5755-5772" color="#ffffff" type="standard"/><Segment name="-35" range="5773-5778" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3817" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="4420" maxFusedRunOn="4420" detectionMode="exactProteinMatch"><Segment range="3767-3820" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7722-7826" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1804-1921" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5387-5508" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="458-583" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5527-5662" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-414" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2011-2214" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2484-2695" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5082-5315" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1076-1309" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2708-2976" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4421-5008" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6102-6690" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="4" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3821-4420" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="4420" maxFusedRunOn="4420"><Segment range="3035-3697" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7652" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6861-7652" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7653-7721" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>b7d43246-82cd-4e90-9e35-6c555d64a81a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.6.7</Created>
<LastModified UTC="8:45:54">2022.6.1</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <StrandColors><TopStrand><ColorRange range="0..2319" colors="black"/><ColorRange range="2422..8324" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2319" colors="black"/><ColorRange range="2422..8324" colors="black"/></BottomStrand></StrandColors>  9ö     i   {         {s"K¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆu•À»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒü]øTÅ@WÑ;»Ð{ŽÌÜ5Äx!'=UªLäPDF1-&”ÀZFÌ`V°¥@EU™ZUÙ’`54ä\k4¥¾ŠEvÑC‰Yà9×ÛúÑÐm^‘¶™–t“_îhˆÛT•G}AN¤uQ5]T”}ótÔÃÞ­�˜@u E!i™Y•$•ZÚA$ßßPÕD‚4DUQ4€Yê+è
èÓAMæ4ûXs‡	Ðôu¦‹‚d�4Ñu¨ášÍtÛŠ5íÒàµ4ïCQoƒˆùPmtï€€(î^Ú�÷Ž#è9(Y[¸F�mÜ~€tEiu§u:YÞúZ[5_MEQauš¤e`5* Z
—eÕ â	™VšÙa’A‚B¥J¨âz¹’D¤4k}¦Ê´~ýÄüt?EP�ÑÚÆ’ìp›
^©TU €t� TY$vY5ÑÙÕåE§UuiÕZÕj›äšcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'*fSDì©!|´løÏosŠ¡ûÙk‘9>Åì³ßìIýœr~c^›é†'á¦99¾˜býsdþ1›Ó0óŒlïü´€Ñ3vÏÝ/ñ  @,5¡p#äE3 •à!‹"x;Ïª¿ É™HÒK<…Êê¬×£ÏEÝ8S¬@;6îúÜÏÏ?Ÿ„<ö÷<(K2¸óïÏˆ#ï…ã/<¹	J•;BÄ|¨¼3¿ OŸ‘í<;½Î£áþ:;^ç.¢¬ªâ¸Ï)Ñ½N!ðÏœäsß`DÇ0øüölHómðßápÈ±8 0Á7ÿS@óaõÔaDßT3ø~´ÿŸŽÜ†œ:çÞ°ßŸôÃœEÿ¯�ÏmuÃw×6ÄRq28Ü„ëN|çðó!°Ç­BðëüAÓ!ºßFÛÍ5Ï|�7ãØeÈà€Ó¬üß­>30ç�:È t÷czÔLö÷ý6øÑpýŠÏ¯<ÈN7MwÄà6È}7>µý³ë=ó;K/ZãÈ7Ïðï|³4AK®]Œ!ÈE¿æu?pŒû=üÇ¿Ð<œßìóÌ¬ñçü÷3ÝòÆð|÷lL|N5ºûs2ÀìvÃPˆ0qÃ<Â.ÜðDËÀ"³Äl?3`ñ(L+ðàó4×F¾ó|?POÌ‚¾|°Ë³ñ÷Ð5à4Ï,Žÿ�<LÏ �Ï¯�¼Ïó_¿ëœL;·Ë|ñË�ÅÌß‚6ÍOÂ>µû�§U¨…F‡‚k¥æ(ïªs˜�œã©cê&ØæŽ¿&­ªï¾‰•¾
8»©„ˆ?(´5È2�â*8s_ýà £ß~-;êð¬êÿïæ�pÁ{lóãèüã�ÐÃŠxu¯ Š,N¾¯`ã³â(÷àkŒÀrúãœ¿¬?µ½0~¾¿0øãÃ¸Ãî¼Ë¼úÏíKïê>ÿ>	ú”}aéÔ4âFé=ê¥QT –·¿\U”n:¨èžîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“I$­‹nškI©—£TVm!^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàîNoÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ÃîšôRHAª�‘ê�Ãõk%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡.Q_i¦Øv—æUd2mÄ?«.ÌÊ°†!WîØÓ¿p·N‹‰LðGSòÐ3-0"ƒ,ƒ¥æ0ÛDÑ¢xD�öc…gÍÑŒ”V|ÛC=¬ö´8Íx l#î³šsÓƒ Ž5iJ\ô
¯ÒÜr‰W=#Ô8           •  —  ñ  	   	  	l  	q  	€  	‡  	‰   z  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  -n<?xml version="1.0"?><Features recycledIDs="0,1" nextValidID="26"><Feature recentID="25" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2412" maxFusedRunOn="2412" detectionMode="exactProteinMatch"><Segment range="2323-2349" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2412" maxFusedRunOn="2412" detectionMode="exactProteinMatch"><Segment range="2353-2379" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2412" maxFusedRunOn="2412" detectionMode="exactProteinMatch"><Segment range="2383-2409" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4881" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4498" maxFusedRunOn="4498" visible="0" detectionMode="exactProteinMatch"><Segment range="4882-4893" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="5714-5730" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="5783-5804" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="5738-5744" color="#ffffff" type="standard"/><Segment range="5745-5762" color="#ffffff" type="standard"/><Segment name="-35" range="5763-5768" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3807" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="4410" maxFusedRunOn="4410" detectionMode="exactProteinMatch"><Segment range="3757-3810" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7712-7816" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1804-1921" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5377-5498" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="458-583" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5517-5652" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-414" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2011-2214" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2474-2685" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5072-5305" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1076-1309" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2698-2966" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4411-4998" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6092-6680" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="4" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3811-4410" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="4410" maxFusedRunOn="4410"><Segment range="3025-3687" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7642" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6851-7642" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7643-7711" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>b7d43246-82cd-4e90-9e35-6c555d64a81a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.6.7</Created>
<LastModified UTC="8:44:40">2022.6.1</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <StrandColors><TopStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="2412..8314" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="2412..8314" colors="black"/></BottomStrand></StrandColors>  <�     
1  'z        'zs"K¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆu•À»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒü]øTÅ@WÑ;»Ð{ŽÌÜ5Äx!'=UªLäPDF1-&”ÀZFÌ`V°¥@EU™ZUÙ’`54ä\k4¥¾ŠEvÑC‰Yà9×ÛúÑÐm^‘¶™–t“_îhˆÛT•G}AN¤uQ5]T”}ótÔÃÞ­�˜@u E!i™Y•$•ZÚA$ßßPÕD‚4DUQ4€Yê+è
èÓAMæ4ûXs‡	Ðôu¦‹‚d�4Ñu¨ášÍtÛŠ5íÒàµ4ïCQoƒˆùPmtï€€(î^Ú�÷Ž#è9(Y[¸F�mÜ~€tEiu§u:YÞúZ[5_MEQauš¤e`5* Z
—eÕ â	™VšÙa’A‚B¥J¨âz¹’D¤4k}¦Ê´~ýÄüt?EP�ÑÚÆ’ìp›
^©TU €t� TY$vY5ÑÙÕåE§UuiÕZÕj›äšcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'*fSDì©!|´løÏosŠ¡ûÙk‘9>Åì³ßaÛ0F
·ývÞ�nøáôA,`Òª“Pý%_àG­î.)VâŸÝµÑw€y‡î`€J�zƒ…à­&��wØ8TEb›µ'ó…â4Ÿ¸T ¯`·¢àUÑã‡P56:û+Ñ=ÿzÊîò3x!ÇT\pÔ½RÜ¶Î-áYÇ( :‡»‡Gà�6�Eý‚s€Ý/�;SOû]×²mcŽ¡SÀ¬D¥588GÝRÔÐ: J„s½5ÐXÕë¹“ÅQAÑEYUÕ]Õ]¾?¨mŸ@²¸Ø`ýa>*á§icWm¶>¡R5Ö½_ÕQ)Ú=íC��p…‡®"½^ìKh¶uAHMX‹Rx>IŒíQg ¶2)ËTT�?açzœDí^îP°u;žôwDEòàÒ}êÁø=`Èù!þ"8¾½÷SAl��âÈ+xÓ8ƒÁÐ_­¢¾š¶±Pˆ!Û¯•}­#ôBÀ/GE7Ið©¹Ø8½7�‘ãLðlAÑTÕEPvgÖiI¯ �ôu †Ñ/ø÷øßõÏˆÑX@AÓ?ærí�4Ñ«G±'öqÉøi�zo¦Ÿ†˜äæúa‹ôyÍ�øÆoLÃÌPn1³¼#òÒDÍÛ?t¿ÄR€ °Ö…À�‘ ÌW€T†,‰àï>ªü@€_&e#I,ò+ª³\
�=táN± ìÛ»ës?<þ~óÛÜð¡,ÊãÏ¿> �¾Œ¼òä%*Tíð>¢ðÎþ�>~G´ðî÷:�‡øèí{œºŠ²«Šã<§Fõ8‡Ã>s‘Ï}�DÃãóØ±#Í·Ã…Ã JÄà€Ã@ßýMÍ‡×Q…}PÏáúÓþ~8OrpëŸzÃ~Óqþ¾7=µ×9ß]üÛIÄ0Èãr­9óœCÃÌ†Ãµ 7Ã¯ñL†ë|uo4×=òß�ÿ`1—#‚N³ó~´øÌpÃžtë �ÓÝ�ëQ3ÛßôÛãEÀö+>¼ó A8Ý5ß€Û IôÜúÔöÏ¬ôoÌí,½k� pß<Ã½ðnÌ@Ñ.¹v0‡ Aü?˜|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øV×î�V¢	®—˜£¾©ÎbsŽ¥�¨›cš:ü˜:¶«¾ú&Vø(âî¦J ü¢Ð× È"7ˆ¨áÍ÷‚€6�}ø´ï«Àz³«ÿ¿š=Ãí³Ï�£óŽ@;(áÖ¼‚(°8rú½ƒŽÏˆ£ß�®3ËëŽrþ°þÖôÁúúüÃã�ãºó.óë?´q,¿¨<ûüø'êQõ‡§PÓ‰¤÷ª•EP xZÞýqVQ¸ê£¢x»‚‘ônà»ƒ—�÷Ž”ûYS£èHëRHˆü¸¢!î	y¿´ªKH�VîM$’¶-ºi­&¦^�QXY´D„Az*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹ƒ¹9¿?åï?Jóö»ÏöZª¦¦4CÄ<û¸NkÑI!:ª>Gª4GÕ¬–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~¹E}¦›aÚ_™U�É·þ¬»3*Âp…_¸wcNüÂÝ:.%0cÀMOË@Ì´ÀŠ°—˜ÃmF‰á7ÙŒ"Ÿ7F2PEYómö³ÚÐã5â€P1°ŒºÎiÏNT b8Ô1¥(	sÐ*¿KqÊ%\pô�S           •  —  ñ  	   	  	  
  k  p    †  ˆ  'y  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ./<?xml version="1.0"?><Features recycledIDs="1" nextValidID="26"><Feature recentID="25" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4203" maxFusedRunOn="4203" detectionMode="exactProteinMatch"><Segment range="4114-4140" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4203" maxFusedRunOn="4203" detectionMode="exactProteinMatch"><Segment range="4144-4170" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4203" maxFusedRunOn="4203" detectionMode="exactProteinMatch"><Segment range="4174-4200" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6672" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6289" maxFusedRunOn="6289" visible="0" detectionMode="exactProteinMatch"><Segment range="6673-6684" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7505-7521" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7574-7595" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="7529-7535" color="#ffffff" type="standard"/><Segment range="7536-7553" color="#ffffff" type="standard"/><Segment name="-35" range="7554-7559" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5598" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="6201" maxFusedRunOn="6201" detectionMode="exactProteinMatch"><Segment range="5548-5601" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9503-9607" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1804-1921" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7168-7289" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="458-583" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7308-7443" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-414" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2011-2214" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4265-4476" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6863-7096" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1076-1309" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4489-4757" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6202-6789" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7883-8471" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="4" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5602-6201" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="6201" maxFusedRunOn="6201"><Segment range="4816-5478" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="9433" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="8642-9433" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="9434-9502" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="Kozak sequence" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2311-2316" color="#a6acb3" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>b7d43246-82cd-4e90-9e35-6c555d64a81a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.6.7</Created>
<LastModified UTC="8:42:15">2022.6.1</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	<StrandColors><TopStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="4203..10105" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="4203..10105" colors="black"/></BottomStrand></StrandColors>  >&     
†  (Í        (Ís"K¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆu•À»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒü]øTÅ@WÑ;»Ð{ŽÌÜ5Äx!'=UªLäPDF1-&”ÀZFÌ`V°¥@EU™ZUÙ’`54ä\k4¥¾ŠEvÑC‰Yà9×ÛúÑÐm^‘¶™–t“_îhˆÛT•G}AN¤uQ5]T”}ótÔÃÞ­�˜@u E!i™Y•$•ZÚA$ßßPÕD‚4DUQ4€Yê+è
èÓAMæ4ûXs‡	Ðôu¦‹‚d�4Ñu¨ášÍtÛŠ5íÒàµ4ïCQoƒˆùPmtï€€(î^Ú�÷Ž#è9(Y[¸F�mÜ~€tEiu§u:YÞúZ[5_MEQauš¤e`5* Z
—eÕ â	™VšÙa’A‚B¥J¨âz¹’D¤4k}¦Ê´~ýÄüt?EP�ÑÚÆ’ìp›
^©TU €t� TY$vY5ÑÙÕåE§UuiÕZÕj›äšcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'*fSDì©!|´løÏosŠ¡ûÙk‘9>Åì³ßb‡^¡Oj"­ DÕaeÕ0;ê®úGZÒv’­u4^abŠ€z�_Ud­­^Ú¥eêÙtXÖ×†m¤fî­6¶ªVÑŠ­]P6S{—Oé…Gž¦9Txª¡íSKº/â†Vá†…%~–ØRïXQŠéõu_WÉ´ÒE^.X–¿„†"‘�ªÕLýÚ¾I5æ­ÓX�Ö�U.}Î¡UÇ†*O�©m;ˆ]˜aOQ¶4™Iùâ´ºÜöHS [ûEÞj‚4zŽ!ýÉµ„ˆa�«J¢×Š¤CXØÔX­Šç­y(m5*Ybëv�J!…"hF7ÆŽéDàF>„¤tj¸ªO]Ø¨Ry8ößºg¾4” ¾FY½€‹êQe†×f�/�QûÒ}– nëP�b5êÇn+ERº©¾Ú¦¡Wè:±ø ©`~*aDY$A¾þWaUÚææºÙéî»µ¶.ucÑuEÒ²)ƒ’;‡‚›­Þjë…^š4™­ª:Zz¿àïª[N¸ÕQÛm!8áÛF…:u8Ùêz.Uèaž.n“è·¿tãÌãÛ-ÙÕ�PƒD#„Óë€BëµAˆÞ�wZmš¡[E�Þ]µ#Ÿz¸buëŽtfµD“ˆæ"¯Fã¦Š€­OÑ¾ŽaV0Ú…�`*¶!ºYx`uy�F4·j¡G•nkIî¾(ã‰X�Sw^ÄŸÙÇ'á¦5é¾˜b~c“›é†/Ñç6@oã½31A¸ÆÎð�ËH7lýÒÿJ ÃZ>DPƒ0	^R²'ƒ¼ú«ñ |™”�$³È\®ªÍp*<ô]Ó…:Ä³nï­ÌüóùøCÏosÂ„³+�>üø‚>ø^2óË�”©S´,GÀú‹Ã;úùùÓÃ»Üê>ã£µîrê*Ê®+Œò�ÔâùÎG=öLq�Ï`FÄ�6ßþ�+‚õ46]FMõC?‡ëOùøá=È`9Ã®}ëùÿL9Ä_úøÜö×\0ç}wólE'pÃ#�ÈN´çÎq2zÔ €ß¿Ä2­ñÔm¼Ó\÷È~7ý€Æ\Ž:ÏÍúÓã1ÃyÓ¬‚Ov7­DÏoÓo� ?Ø¬úóÌ�ãt×|Nl�'ÓsëQÿÛ>³Ñ¿3´²õ®<�Ã|ð÷Á»1DºåØÂ�[ðþañ'S÷Ï³ßÌqËýÉÍþÏ<ÊÏÏs=ß,oÏvÄÀ·ÄãQ{¯·3,Çl5€³3Ì Âà}ÏL±L Â+<FÃó6„Â¿3Mt`PûàÏ7ÃõüÈ+à÷Ë»?}P.LòÈïðHÃÄÌðüúøûÌÿ5ûþ¹ÄÃ°ë|·Ï±üP|Íø#aüÔü#á[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢mŽhëò`êÚ®ûè™[à£‹º˜A(ƒò‹C\ƒ ˆÞ"£‡5ÿÞ
 Ú=÷âÓ¾¯êÎ¯þþh÷¶Ï>>�Î8 ì8 7‡Zò¢ÀáËêö;>"�~¸Ì/®9ËúÃû[ÓëëóŽ<;Œ>ëÌ»Ï¬þÑÄ°nþ óïóàŸ©GÖ�CN$n“ÞªU@àP9k{õÅYFãªŽ‰à.î
GÑ¸O€Nî^@_Þ:SíeN�¡#­I"#òâˆ‡¸%äFþÑþ©-"[¹4’JØ¶é¦´š™z5EafÑè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.äæüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóì>á9¯E$„ê¨ù¨Ñ?V²[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=øråöšm‡i~eVC&ÜCú²ìÌ«aÂ~áÝ�;ðGtè¸”Á�4u?-2Ó(0"À8:^c´M'„Hßf0ˆV|ÝÉAgÍ´3ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ=N            •  —  ñ  	   	  	  ]  ¾  Ã  Ò  Ù  Û  (Ì  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  /<?xml version="1.0"?><Features nextValidID="26"><Feature recentID="25" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4542" maxFusedRunOn="4542" detectionMode="exactProteinMatch"><Segment range="4453-4479" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4542" maxFusedRunOn="4542" detectionMode="exactProteinMatch"><Segment range="4483-4509" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4542" maxFusedRunOn="4542" detectionMode="exactProteinMatch"><Segment range="4513-4539" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7011" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6628" maxFusedRunOn="6628" visible="0" detectionMode="exactProteinMatch"><Segment range="7012-7023" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7844-7860" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7913-7934" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="7868-7874" color="#ffffff" type="standard"/><Segment range="7875-7892" color="#ffffff" type="standard"/><Segment name="-35" range="7893-7898" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5937" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="6540" maxFusedRunOn="6540" detectionMode="exactProteinMatch"><Segment range="5887-5940" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9842-9946" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1804-1921" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7507-7628" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="458-583" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7647-7782" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-414" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2011-2214" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4604-4815" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7202-7435" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1076-1309" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4828-5096" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6541-7128" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="8222-8810" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="4" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5941-6540" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="6540" maxFusedRunOn="6540"><Segment range="5155-5817" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="9772" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="8981-9772" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="9773-9841" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="FASTKD2" directionality="1" translationMW="81464.93" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4542" maxFusedRunOn="4542"><Segment range="2317-4446" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="Kozak sequence" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2311-2316" color="#a6acb3" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>b7d43246-82cd-4e90-9e35-6c555d64a81a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.6.7</Created>
<LastModified UTC="8:39:53">2022.6.1</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	<StrandColors><TopStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="4542..10444" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="4542..10444" colors="black"/></BottomStrand></StrandColors>  =@     
|  (Ç        (Çs"K¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆu•À»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒü]øTÅ@WÑ;»Ð{ŽÌÜ5Äx!'=UªLäPDF1-&”ÀZFÌ`V°¥@EU™ZUÙ’`54ä\k4¥¾ŠEvÑC‰Yà9×ÛúÑÐm^‘¶™–t“_îhˆÛT•G}AN¤uQ5]T”}ótÔÃÞ­�˜@u E!i™Y•$•ZÚA$ßßPÕD‚4DUQ4€Yê+è
èÓAMæ4ûXs‡	Ðôu¦‹‚d�4Ñu¨ášÍtÛŠ5íÒàµ4ïCQoƒˆùPmtï€€(î^Ú�÷Ž#è9(Y[¸F�mÜ~€tEiu§u:YÞúZ[5_MEQauš¤e`5* Z
—eÕ â	™VšÙa’A‚B¥J¨âz¹’D¤4k}¦Ê´~ýÄüt?EP�ÑÚÆ’ìp›
^©TU €t� TY$vY5ÑÙÕåE§UuiÕZÕj›äšcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'*fSDì©!|´løÏosŠ¡ûÙk‘9>Åì¶(uêö R*ÒMV]S¾ªà_¤u­'i*×SEææ(¨¨ÕõVJÚÕà]ªV^­—E�mxfÚFnêÐckj¥m êÕÕe7¹tþ‘hTyêc•GŠªÕ4»¢þ(enahQRWém….õ…®ŸPWUõ|›M$Uâá‰køHb)Ú­TÏÝ«ä“^jÝ5ˆhõQâá‡ÑLê\xb¤÷øÚ–Ó¸…Ù†õcI”Ÿž+K­Ïd…:¿´]æ¨#G¨áÐ\›XH†˜ú´ª-xªD5�}�EŠØ®qpÑg’†ÓR U‘F.·iÔ¢R&�Tc|hî”A^aSè@ZGF«Š¡dõÝŠ…'“�amñ»¦{àacIBäe›Ø¾¥XmvhÒøÕ½'ÙjîµèÑ¦ ã^¬vàR´U+ª›íªj~ƒ«Š
–â¦E’Dáoåv]®nk­žžë»[bçV=T]+ R˜9#¸x)ºÝæ®¸Ué cIšÚ£¡…§«þú¥´ë�U¶ÒŽ´aØQc§S�ž g¢å^†âæáÙ>‹{÷N<ÀN=²Ý�XÕ4B8M>¸!AÞ»T�è÷u¦Ù¡ª´XåÐ[R9÷«† g^¸çFkTI8Žb*ôn:h¨
Ô÷ýkèæc¨XÖ«b µ—€FW‘cKvªyVæ´‘NëâŽ8•ˆÕ7uìIýœr~c^›é†'á¦99¾˜býsdþ1›Ó0óŒlïü´€Ñ3vÏÝ/ñ  @,5¡p#äE3 •à!‹"x;Ïª¿ É™HÒK<…Êê¬×£ÏEÝ8S¬@;6îúÜÏÏ?Ÿ„<ö÷<(K2¸óïÏˆ#ï…ã/<¹	J•;BÄ|¨¼3¿ OŸ‘í<;½Î£áþ:;^ç.¢¬ªâ¸Ï)Ñ½N!ðÏœäsß`DÇ0øüölHómðßápÈ±8 0Á7ÿS@óaõÔaDßT3ø~´ÿŸŽÜ†œ:çÞ°ßŸôÃœEÿ¯�ÏmuÃw×6ÄRq28Ü„ëN|çðó!°Ç­BðëüAÓ!ºßFÛÍ5Ï|�7ãØeÈà€Ó¬üß­>30ç�:È t÷czÔLö÷ý6øÑpýŠÏ¯<ÈN7MwÄà6È}7>µý³ë=ó;K/ZãÈ7Ïðï|³4AK®]Œ!ÈE¿æu?pŒû=üÇ¿Ð<œßìóÌ¬ñçü÷3ÝòÆð|÷lL|N5ºûs2ÀìvÃPˆ0qÃ<Â.ÜðDËÀ"³Äl?3`ñ(L+ðàó4×F¾ó|?POÌ‚¾|°Ë³ñ÷Ð5à4Ï,Žÿ�<LÏ �Ï¯�¼Ïó_¿ëœL;·Ë|ñË�ÅÌß‚6ÍOÂ>µû�§U¨…F‡‚k¥æ(ïªs˜�œã©cê&ØæŽ¿&­ªï¾‰•¾
8»©„ˆ?(´5È2�â*8s_ýà £ß~-;êð¬êÿïæ�pÁ{lóãèüã�ÐÃŠxu¯ Š,N¾¯`ã³â(÷àkŒÀrúãœ¿¬?µ½0~¾¿0øãÃ¸Ãî¼Ë¼úÏíKïê>ÿ>	ú”}aéÔ4âFé=ê¥QT –·¿\U”n:¨èžîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“I$­‹nškI©—£TVm!^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàîNoÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ÃîšôRHAª�‘ê�Ãõk%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡.Q_i¦Øv—æUd2mÄ?«.ÌÊ°†!WîØÓ¿p·N‹‰LðGSòÐ3-0"ƒ,ƒ¥æ0ÛDÑ¢xD�öc…gÍÑŒ”V|ÛC=¬ö´8Íx l#î³šsÓƒ Ž5iJ\ô
¯ÒÜr‰W=#Ô8           •  —  ñ  	   W  ¸  ½  Ì  Ó  Õ  (Æ  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  .¢<?xml version="1.0"?><Features nextValidID="25"><Feature recentID="0" name="FASTKD2" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4536" maxFusedRunOn="4536"><Segment range="2311-4440" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="24" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="9766" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="8975-9766" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="9767-9835" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="6534" maxFusedRunOn="6534"><Segment range="5149-5811" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5935-6534" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="8216-8804" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="20" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6535-7122" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4822-5090" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1076-1309" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7196-7429" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4598-4809" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2011-2214" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-414" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7641-7776" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="458-583" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7501-7622" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1804-1921" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9836-9940" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5931" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="6534" maxFusedRunOn="6534" detectionMode="exactProteinMatch"><Segment range="5881-5934" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="7862-7868" color="#ffffff" type="standard"/><Segment range="7869-7886" color="#ffffff" type="standard"/><Segment name="-35" range="7887-7892" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7907-7928" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7838-7854" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7005" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6622" maxFusedRunOn="6622" visible="0" detectionMode="exactProteinMatch"><Segment range="7006-7017" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4536" maxFusedRunOn="4536" detectionMode="exactProteinMatch"><Segment range="4507-4533" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4536" maxFusedRunOn="4536" detectionMode="exactProteinMatch"><Segment range="4477-4503" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4536" maxFusedRunOn="4536" detectionMode="exactProteinMatch"><Segment range="4447-4473" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>cf3c1581-de24-489b-8601-ed8aa81d943f</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.6.7</Created>
<LastModified UTC="5:47:47">2022.3.30</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	<StrandColors><TopStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="4536..10438" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="4536..10438" colors="black"/></BottomStrand></StrandColors>  ;¯     ð  "—        "—s"K¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆu•À»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒü]øTÅ@WÑ;»Ð{ŽÌÜ5Äx!'=UªLäPDF1-&”ÀZFÌ`V°¥@EU™ZUÙ’`54ä\k4¥¾ŠEvÑC‰Yà9×ÛúÑÐm^‘¶™–t“_îhˆÛT•G}AN¤uQ5]T”}ótÔÃÞ­�˜@u E!i™Y•$•ZÚA$ßßPÕD‚4DUQ4€Yê+è
èÓAMæ4ûXs‡	Ðôu¦‹‚d�4Ñu¨ášÍtÛŠ5íÒàµ4ïCQoƒˆùPmtï€€(î^Ú�÷Ž#è9(Y[¸F�mÜ~€tEiu§u:YÞúZ[5_MEQauš¤e`5* Z
—eÕ â	™VšÙa’A‚B¥J¨âz¹’D¤4k}¦Ê´~ýÄüt?EP�ÑÚÆ’ìp›
^©TU €t� TY$vY5ÑÙÕåE§UuiÕZÕj›äšcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'*fSDì©!|´løÏosŠ¡ûÙk‘9>Åì¶<Ì²?DSR±JªANÌH„iIÜ´ÃýÄ=Î½><Ç;lêˆ=Ð_[‹»Òª¼=84]1Õß“ÄI‹‘QÐÔ&j;öá`Kˆ¦àT8½Qà`˜.¨aÄý4ci7]¨QÁÌZ…Ð»l�D«tãƒIx5uÔìIýœr~c^›é†'á¦99¾˜býsdþ1›Ó0óŒlïü´€Ñ3vÏÝ/ñ  @,5¡p#äE3 •à!‹"x;Ïª¿ É™HÒK<…Êê¬×£ÏEÝ8S¬@;6îúÜÏÏ?Ÿ„<ö÷<(K2¸óïÏˆ#ï…ã/<¹	J•;BÄ|¨¼3¿ OŸ‘í<;½Î£áþ:;^ç.¢¬ªâ¸Ï)Ñ½N!ðÏœäsß`DÇ0øüölHómðßápÈ±8 0Á7ÿS@óaõÔaDßT3ø~´ÿŸŽÜ†œ:çÞ°ßŸôÃœEÿ¯�ÏmuÃw×6ÄRq28Ü„ëN|çðó!°Ç­BðëüAÓ!ºßFÛÍ5Ï|�7ãØeÈà€Ó¬üß­>30ç�:È t÷czÔLö÷ý6øÑpýŠÏ¯<ÈN7MwÄà6È}7>µý³ë=ó;K/ZãÈ7Ïðï|³4AK®]Œ!ÈE¿æu?pŒû=üÇ¿Ð<œßìóÌ¬ñçü÷3ÝòÆð|÷lL|N5ºûs2ÀìvÃPˆ0qÃ<Â.ÜðDËÀ"³Äl?3`ñ(L+ðàó4×F¾ó|?POÌ‚¾|°Ë³ñ÷Ð5à4Ï,Žÿ�<LÏ �Ï¯�¼Ïó_¿ëœL;·Ë|ñË�ÅÌß‚6ÍOÂ>µû�§U¨…F‡‚k¥æ(ïªs˜�œã©cê&ØæŽ¿&­ªï¾‰•¾
8»©„ˆ?(´5È2�â*8s_ýà £ß~-;êð¬êÿïæ�pÁ{lóãèüã�ÐÃŠxu¯ Š,N¾¯`ã³â(÷àkŒÀrúãœ¿¬?µ½0~¾¿0øãÃ¸Ãî¼Ë¼úÏíKïê>ÿ>	ú”}aéÔ4âFé=ê¥QT –·¿\U”n:¨èžîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“I$­‹nškI©—£TVm!^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàîNoÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ÃîšôRHAª�‘ê�Ãõk%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡.Q_i¦Øv—æUd2mÄ?«.ÌÊ°†!WîØÓ¿p·N‹‰LðGSòÐ3-0"ƒ,ƒ¥æ0ÛDÑ¢xD�öc…gÍÑŒ”V|ÛC=¬ö´8Íx l#î³šsÓƒ Ž5iJ\ô
¯ÒÜr‰W=#Ô8           •  —  ñ  	   '  ˆ  �  œ  £  ¥  "–  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  .Ÿ<?xml version="1.0"?><Features nextValidID="25"><Feature recentID="24" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="8182" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="7391-8182" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="8183-8251" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="4950" maxFusedRunOn="4950"><Segment range="3565-4227" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4351-4950" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6632-7220" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="20" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4951-5538" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3238-3506" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1076-1309" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5612-5845" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3014-3225" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2011-2214" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-414" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6057-6192" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="458-583" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5917-6038" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1804-1921" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8252-8356" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4347" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="4950" maxFusedRunOn="4950" detectionMode="exactProteinMatch"><Segment range="4297-4350" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="6278-6284" color="#ffffff" type="standard"/><Segment range="6285-6302" color="#ffffff" type="standard"/><Segment name="-35" range="6303-6308" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6323-6344" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6254-6270" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5421" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5038" maxFusedRunOn="5038" visible="0" detectionMode="exactProteinMatch"><Segment range="5422-5433" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2952" maxFusedRunOn="2952" detectionMode="exactProteinMatch"><Segment range="2923-2949" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2952" maxFusedRunOn="2952" detectionMode="exactProteinMatch"><Segment range="2893-2919" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2952" maxFusedRunOn="2952" detectionMode="exactProteinMatch"><Segment range="2863-2889" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="CBFB" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2952" maxFusedRunOn="2952"><Segment range="2311-2856" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>cf3c1581-de24-489b-8601-ed8aa81d943f</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.6.7</Created>
<LastModified UTC="5:46:44">2022.3.30</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <StrandColors><TopStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="2952..8854" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="2952..8854" colors="black"/></BottomStrand></StrandColors>  A‚     Ž  -        -s"K¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆu•À»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒü]øTÅ@WÑ;»Ð{ŽÌÜ5Äx!'=UªLäPDF1-&”ÀZFÌ`V°¥@EU™ZUÙ’`54ä\k4¥¾ŠEvÑC‰Yà9×ÛúÑÐm^‘¶™–t“_îhˆÛT•G}AN¤uQ5]T”}ótÔÃÞ­�˜@u E!i™Y•$•ZÚA$ßßPÕD‚4DUQ4€Yê+è
èÓAMæ4ûXs‡	Ðôu¦‹‚d�4Ñu¨ášÍtÛŠ5íÒàµ4ïCQoƒˆùPmtï€€(î^Ú�÷Ž#è9(Y[¸F�mÜ~€tEiu§u:YÞúZ[5_MEQauš¤e`5* Z
—eÕ â	™VšÙa’A‚B¥J¨âz¹’D¤4k}¦Ê´~ýÄüt?EP�ÑÚÆ’ìp›
^©TU €t� TY$vY5ÑÙÕåE§UuiÕZÕj›äšcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'*fSDì©!|´løÏosŠ¡ûÙk‘9>Åì¶ tÂ.ðsäÓïCÀü�ûÀ{þø ,�Ìp†OBbßsÇ�M8ß< ÿµûŒCâ5+Ý7ØÚa€Ø¼ë¯ýÔ;ˆàü"÷ð¶?<Íôÿ4�èADî `Õ |MÐøW»ÔQSPAÜSPXë{…j…ˆ¡²ý=…RÞ Ž8øš…A@Ú3€Õ vçà}/]ßø¿·ëùÐ¬ wŸ¬‹TÿOSàF5@ØP{Ô¿„¨ñ®0ôàÿÏàLˆýÏàvè?àóÏ5bÁ+àM¿ÀÐOÿsûÓŽî?L4Ò7×x1Ó�Eð0ô³­0ê­;Ãý7Ó> ð‘Ô`M858ÝùÓî°8ý?À3¬}‡¾q…A„cãá80¶†aáü0Ò=ôÐC€N8ÝÁ¾vÐWŒmµø¯ˆãï…øÔK@ãˆ„ CÏŠÓ]¿lÍ8ß48€äÈ`ÏàEÃ713ŽtüëíPëQŠÃ³ºÔþvóž6@ã`IØÌ>8ÌuÇî¼�· 0E7÷HôC…8Lv8=_÷ÓßR8ãµ,#„|A<Ìõ<²Ïa°ï Lëo}È\ÈØÃL40àÃÈ!<ÛùÄ �SM1Å u;áAjã~¡Ó?oàÏ¿Ás8ã� O„P@5‰ã¯ºÜŽ(!õM!EÐ-´=ÿŽÈFˆ�9À€¾¾ún~­Ò8��Êô= ô>ˆò}©Ö?Eôã]8ÈÝÿÐÒÓOøÑ>AAAAPÄ Þ´ãÓ¿çÛcSL4ÿí7ˆûF÷ƒØ(‚DøÜâûÿ±ÓêÓ¿K†û»M÷�¶ãÜýô„ã�øÂ;ã½�î>pßîÿ_¾�G¨ƒýÖ’>ÀÿD¿ÜJúÿ½þÏòï<óâôâ„ãóýøíK”¼CÓï½8àÐý€wû�ý<ÓâÑ;�ƒŠôÿBi´ÿ´à?N£ÄþOàÓà9,8ø<~4;÷AD"~?°îýôøOýôî?MÐS‡ø?NçÅ´Ž:^÷;xû/±'öqÉøi�zo¦Ÿ†˜äæúa‹ôyÍ�øÆoLÃÌPn1³¼#òÒDÍÛ?t¿ÄR€ °Ö…À�‘ ÌW€T†,‰àï>ªü@€_&e#I,ò+ª³\
�=táN± ìÛ»ës?<þ~óÛÜð¡,ÊãÏ¿> �¾Œ¼òä%*Tíð>¢ðÎþ�>~G´ðî÷:�‡øèí{œºŠ²«Šã<§Fõ8‡Ã>s‘Ï}�DÃãóØ±#Í·Ã…Ã JÄà€Ã@ßýMÍ‡×Q…}PÏáúÓþ~8OrpëŸzÃ~Óqþ¾7=µ×9ß]üÛIÄ0Èãr­9óœCÃÌ†Ãµ 7Ã¯ñL†ë|uo4×=òß�ÿ`1—#‚N³ó~´øÌpÃžtë �ÓÝ�ëQ3ÛßôÛãEÀö+>¼ó A8Ý5ß€Û IôÜúÔöÏ¬ôoÌí,½k� pß<Ã½ðnÌ@Ñ.¹v0‡ Aü?˜|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øV×î�V¢	®—˜£¾©ÎbsŽ¥�¨›cš:ü˜:¶«¾ú&Vø(âî¦J ü¢Ð× È"7ˆ¨áÍ÷‚€6�}ø´ï«Àz³«ÿ¿š=Ãí³Ï�£óŽ@;(áÖ¼‚(°8rú½ƒŽÏˆ£ß�®3ËëŽrþ°þÖôÁúúüÃã�ãºó.óë?´q,¿¨<ûüø'êQõ‡§PÓ‰¤÷ª•EP xZÞýqVQ¸ê£¢x»‚‘ônà»ƒ—�÷Ž”ûYS£èHëRHˆü¸¢!î	y¿´ªKH�VîM$’¶-ºi­&¦^�QXY´D„Az*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹ƒ¹9¿?åï?Jóö»ÏöZª¦¦4CÄ<û¸NkÑI!:ª>Gª4GÕ¬–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~¹E}¦›aÚ_™U�É·þ¬»3*Âp…_¸wcNüÂÝ:.%0cÀMOË@Ì´ÀŠ°—˜ÃmF‰á7ÙŒ"Ÿ7F2PEYómö³ÚÐã5â€P1°ŒºÎiÏNT b8Ô1¥(	sÐ*¿KqÊ%\pô�S           •  —  ñ  	   ž  ÿ          -  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  1<?xml version="1.0"?><Features nextValidID="26"><Feature recentID="25" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5631" maxFusedRunOn="5631" detectionMode="exactProteinMatch"><Segment range="5542-5568" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5631" maxFusedRunOn="5631" detectionMode="exactProteinMatch"><Segment range="5572-5598" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5631" maxFusedRunOn="5631" detectionMode="exactProteinMatch"><Segment range="5602-5628" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="8100" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7717" maxFusedRunOn="7717" visible="0" detectionMode="exactProteinMatch"><Segment range="8101-8112" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="8933-8949" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="9002-9023" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="8957-8963" color="#ffffff" type="standard"/><Segment range="8964-8981" color="#ffffff" type="standard"/><Segment name="-35" range="8982-8987" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="7026" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="7629" maxFusedRunOn="7629" detectionMode="exactProteinMatch"><Segment range="6976-7029" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10931-11035" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1804-1921" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8596-8717" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="458-583" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8736-8871" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-414" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2011-2214" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5693-5904" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8291-8524" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1076-1309" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5917-6185" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7630-8217" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="9311-9899" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="4" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="7030-7629" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="7629" maxFusedRunOn="7629"><Segment range="6244-6906" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10861" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="10070-10861" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10862-10930" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="Shine-Dalgarno sequence" type="RBS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4990-4998" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;full consensus sequence for ribosome-binding sites upstream of start codons in &lt;i&gt;E. coli&lt;/i&gt;; complementary to a region in the 3' end of the 16S rRNA (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/7528374&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/7528374&quot;&gt;Chen et al., 1994&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="PLEKHG5" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5631" maxFusedRunOn="5631"><Segment range="2311-5535" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>cf3c1581-de24-489b-8601-ed8aa81d943f</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.6.7</Created>
<LastModified UTC="5:43:41">2022.3.30</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  ½<StrandColors><TopStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="2310..2516" colors="red"/><ColorRange range="5254..5453" colors="red"/><ColorRange range="5631..11533" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="2310..2516" colors="red"/><ColorRange range="5254..5453" colors="red"/><ColorRange range="5631..11533" colors="black"/></BottomStrand></StrandColors>  <©     	ê  &~        &~s"K¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆu•À»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒü]øTÅ@WÑ;»Ð{ŽÌÜ5Äx!'=UªLäPDF1-&”ÀZFÌ`V°¥@EU™ZUÙ’`54ä\k4¥¾ŠEvÑC‰Yà9×ÛúÑÐm^‘¶™–t“_îhˆÛT•G}AN¤uQ5]T”}ótÔÃÞ­�˜@u E!i™Y•$•ZÚA$ßßPÕD‚4DUQ4€Yê+è
èÓAMæ4ûXs‡	Ðôu¦‹‚d�4Ñu¨ášÍtÛŠ5íÒàµ4ïCQoƒˆùPmtï€€(î^Ú�÷Ž#è9(Y[¸F�mÜ~€tEiu§u:YÞúZ[5_MEQauš¤e`5* Z
—eÕ â	™VšÙa’A‚B¥J¨âz¹’D¤4k}¦Ê´~ýÄüt?EP�ÑÚÆ’ìp›
^©TU €t� TY$vY5ÑÙÕåE§UuiÕZÕj›äšcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'*fSDì©!|´løÏosŠ¡ûÙk‘9>Åì¶
µ¿EžaCQrûíÐN|*¹B:àU(á¢ùB´™Š „4TàtwÝDÄ�E/¤ƒSª‡F„FoF©i“[]1{_•àîÒQ•†ÙçÖiš÷ˆÓ�%
–jQâ´¥b5Ìté®ú7]XQn"¶6&×M˜ùâ[56haI.o}ø�¿QNêD��†’V·=V†A–vvguSh4YŠ']EÕyXƒZ%lh �JEú¯A�¡—©`}H.êhÃ@TºÅÖr>õÖ?£Fè¯‰’‹]„º0ÓàšðJYQ]¶™h�t4Þ[ê÷€!J}‰j÷«Q¾ì]­?X•¹~õ¶ê™¶žŽ%=”?Ókâa›Ÿ´xSõUÖ^Ë­Õ9‚*èY«u%®€¨xE‹YŠX[^WøQ•QúÖhbpÒ� 4Y™yã ”RrhXQX¸>þ4ôUÛ…Ô£™Ê`ªYæú!¾Ûƒ6ªhÔmÕƒQY¸'¨‚’â)Ôª‹g¯oMæŸ±'öqÉøi�zo¦Ÿ†˜äæúa‹ôyÍ�øÆoLÃÌPn1³¼#òÒDÍÛ?t¿ÄR€ °Ö…À�‘ ÌW€T†,‰àï>ªü@€_&e#I,ò+ª³\
�=táN± ìÛ»ës?<þ~óÛÜð¡,ÊãÏ¿> �¾Œ¼òä%*Tíð>¢ðÎþ�>~G´ðî÷:�‡øèí{œºŠ²«Šã<§Fõ8‡Ã>s‘Ï}�DÃãóØ±#Í·Ã…Ã JÄà€Ã@ßýMÍ‡×Q…}PÏáúÓþ~8OrpëŸzÃ~Óqþ¾7=µ×9ß]üÛIÄ0Èãr­9óœCÃÌ†Ãµ 7Ã¯ñL†ë|uo4×=òß�ÿ`1—#‚N³ó~´øÌpÃžtë �ÓÝ�ëQ3ÛßôÛãEÀö+>¼ó A8Ý5ß€Û IôÜúÔöÏ¬ôoÌí,½k� pß<Ã½ðnÌ@Ñ.¹v0‡ Aü?˜|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øV×î�V¢	®—˜£¾©ÎbsŽ¥�¨›cš:ü˜:¶«¾ú&Vø(âî¦J ü¢Ð× È"7ˆ¨áÍ÷‚€6�}ø´ï«Àz³«ÿ¿š=Ãí³Ï�£óŽ@;(áÖ¼‚(°8rú½ƒŽÏˆ£ß�®3ËëŽrþ°þÖôÁúúüÃã�ãºó.óë?´q,¿¨<ûüø'êQõ‡§PÓ‰¤÷ª•EP xZÞýqVQ¸ê£¢x»‚‘ônà»ƒ—�÷Ž”ûYS£èHëRHˆü¸¢!î	y¿´ªKH�VîM$’¶-ºi­&¦^�QXY´D„Az*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹ƒ¹9¿?åï?Jóö»ÏöZª¦¦4CÄ<û¸NkÑI!:ª>Gª4GÕ¬–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~¹E}¦›aÚ_™U�É·þ¬»3*Âp…_¸wcNüÂÝ:.%0cÀMOË@Ì´ÀŠ°—˜ÃmF‰á7ÙŒ"Ÿ7F2PEYómö³ÚÐã5â€P1°ŒºÎiÏNT b8Ô1¥(	sÐ*¿KqÊ%\pô�S           •  —  ñ  	     o  t  ƒ  Š  Œ  &}  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  .Ÿ<?xml version="1.0"?><Features nextValidID="25"><Feature recentID="24" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="9181" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="8390-9181" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="9182-9250" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="5949" maxFusedRunOn="5949"><Segment range="4564-5226" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5350-5949" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7631-8219" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="20" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5950-6537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4237-4505" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1076-1309" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6611-6844" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4013-4224" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2011-2214" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-414" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7056-7191" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="458-583" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6916-7037" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1804-1921" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9251-9355" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5346" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="5949" maxFusedRunOn="5949" detectionMode="exactProteinMatch"><Segment range="5296-5349" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="7277-7283" color="#ffffff" type="standard"/><Segment range="7284-7301" color="#ffffff" type="standard"/><Segment name="-35" range="7302-7307" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7322-7343" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="7253-7269" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6420" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6037" maxFusedRunOn="6037" visible="0" detectionMode="exactProteinMatch"><Segment range="6421-6432" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3951" maxFusedRunOn="3951" detectionMode="exactProteinMatch"><Segment range="3922-3948" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3951" maxFusedRunOn="3951" detectionMode="exactProteinMatch"><Segment range="3892-3918" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3951" maxFusedRunOn="3951" detectionMode="exactProteinMatch"><Segment range="3862-3888" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="FAR1" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3951" maxFusedRunOn="3951"><Segment range="2311-3855" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>cf3c1581-de24-489b-8601-ed8aa81d943f</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.6.7</Created>
<LastModified UTC="5:38:57">2022.3.30</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <StrandColors><TopStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="3951..9853" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="3951..9853" colors="black"/></BottomStrand></StrandColors>  <ë     	v  $°        $°s"K¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆu•À»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒü]øTÅ@WÑ;»Ð{ŽÌÜ5Äx!'=UªLäPDF1-&”ÀZFÌ`V°¥@EU™ZUÙ’`54ä\k4¥¾ŠEvÑC‰Yà9×ÛúÑÐm^‘¶™–t“_îhˆÛT•G}AN¤uQ5]T”}ótÔÃÞ­�˜@u E!i™Y•$•ZÚA$ßßPÕD‚4DUQ4€Yê+è
èÓAMæ4ûXs‡	Ðôu¦‹‚d�4Ñu¨ášÍtÛŠ5íÒàµ4ïCQoƒˆùPmtï€€(î^Ú�÷Ž#è9(Y[¸F�mÜ~€tEiu§u:YÞúZ[5_MEQauš¤e`5* Z
—eÕ â	™VšÙa’A‚B¥J¨âz¹’D¤4k}¦Ê´~ýÄüt?EP�ÑÚÆ’ìp›
^©TU €t� TY$vY5ÑÙÕåE§UuiÕZÕj›äšcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'*fSDì©!|´løÏosŠ¡ûÙk‘9>Åì¶/tôÓÖëMÜÜŠõhO}LW�¿LMÍíâïš¹Æ=üÖ"|Ûm6ÁõmµÓx·¿]‡ë}W¯ÑJÀÒÇK3×t¹Ãà:�¸]6áÝ*<E+ÐRÓÐàôVí;}/OàÏ¿}ÿÄþì´�\?ÃUx¯4ôLá�ü?Ý10åE¢á¼€Ä'H<J«7�MuÔàÐÏã\óÍ?HÓN;L�ñ0ã0 á8í:Ñ÷ÂA à=ÒÎÓ�Nà�_P}kGøQ- óþ8ñ2G´ÑB4�Íc Môã4øCp4LÀÌ€ð4Í8àrÓâ¡8HN2>Ïƒ„8ÏûgŸ†˜×¦úa‰øiŽNo¦¿GœÙ¿ŒfôÌ<Åã;Â?- 4LÝ³÷KüE( h\ùBÀ%xHbÈžóê¯Ä EòfR4’Ï!rº«5À¨óÑwNëÍ»¾·3óÏçá=½Ï
Ì®<ûóâûáxËÏ.BR¥NÐ±ê/ïèçä{Oïs¨øŽŽ×¹Ë¨«*¸®3ÊtoSˆ|3ç9÷Ø1ÄL>?=�<Û|7ø\2¬N0DÿÔÐ<Ø}uQ7Õþ­?çã„÷!€ç¹÷¬7çý0çëãsÛ]pÃ�õßÍ±œAÃŽ7!:ÓŸ9Ä<<Èl1ëP‚|:ÿtÈn·ÇQ¶óMsß Møßör8 4ë?7ëOŒÇ9çN²=ØÞµ=½ÿM¾4\ ÿb³ëÏ2�Ó]ñ8²ŸMÏ­GÿlúÏFüÎÒËÖ¸òóÁü;ßìÄRë—croÃù‡Ä�OÜ#>Ï1Ç/ô'7û<ó+<yÿ=Ì÷|±¼=Ûß�Eî¾ÜÌ°;°Ô"ÌpÏ0ƒ�÷<2Å0¬ñÌØ<J
ü8<Í5Ñ�Cïƒ<ßÔó ¯ƒß,2ìü}ô@¸3Ë#¿Á C3À#óëàGï3ü×ïúçÃ­òß<rÄ#ñAó7à�‡óSð�…m~àiÕj!Q¡àšéyŠ;êœæ g8êXú‰¶9£¯Éƒ«j»ï¢eo‚Ž.êa¢Ê-r‚#xŠŽ×ÿx(h÷ß‹Nú¼«:¿ûù£Ü0^Û<øú?8àt°â€ÞkÈ"‹ S‡/«Ø8ìøŠ=øã0¾¸ç/ëíoL¯¯Ì>8ðî0û¯2ï>³ûGÁ»úƒÏ¿Ï‚~¥Xzu8‘ºOz©TU �@å­ï×eŽª:'€»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒI+bÛ¦šÒjeèÕ…›DHD¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸;“›óþ^óô¯?k¼ÿ`áåªªjcD<CÏ°û„æ½’Cª Cäz£DpýZÉm‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷áË”WÚi¶¥ù•Y›qêË³2¬!‡Uû‡v4ïÁ-Ó¢âS<ÑÔü´ËL À‹ àéyŒ6Ñ4hž#}˜Â!Yótc%UŸ6ÐÏk=­3^(Àû¬æœôàÅ@#�CR€—=«ô·¢UÇHõ8           •  —  ñ  	   @  ¡  ¦  µ  ¼  ¾  $¯  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  .¡<?xml version="1.0"?><Features nextValidID="25"><Feature recentID="24" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3489" maxFusedRunOn="3489" detectionMode="exactProteinMatch"><Segment range="3400-3426" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3489" maxFusedRunOn="3489" detectionMode="exactProteinMatch"><Segment range="3430-3456" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3489" maxFusedRunOn="3489" detectionMode="exactProteinMatch"><Segment range="3460-3486" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5958" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5575" maxFusedRunOn="5575" visible="0" detectionMode="exactProteinMatch"><Segment range="5959-5970" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6791-6807" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6860-6881" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="6815-6821" color="#ffffff" type="standard"/><Segment range="6822-6839" color="#ffffff" type="standard"/><Segment name="-35" range="6840-6845" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4884" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="5487" maxFusedRunOn="5487" detectionMode="exactProteinMatch"><Segment range="4834-4887" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8789-8893" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1804-1921" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6454-6575" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="458-583" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6594-6729" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-414" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2011-2214" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3551-3762" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6149-6382" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1076-1309" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3775-4043" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5488-6075" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="7169-7757" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="3" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4888-5487" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="5487" maxFusedRunOn="5487"><Segment range="4102-4764" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="8719" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="7928-8719" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="8720-8788" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="HOMER3" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3489" maxFusedRunOn="3489"><Segment range="2311-3393" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>cf3c1581-de24-489b-8601-ed8aa81d943f</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.6.7</Created>
<LastModified UTC="5:34:40">2022.3.30</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  »<StrandColors><TopStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="2580..2580" colors="red"/><ColorRange range="3335..3335" colors="red"/><ColorRange range="3489..9391" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="2580..2580" colors="red"/><ColorRange range="3335..3335" colors="red"/><ColorRange range="3489..9391" colors="black"/></BottomStrand></StrandColors>  B÷        +X        +Xs"K¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆu•À»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒü]øTÅ@WÑ;»Ð{ŽÌÜ5Äx!'=UªLäPDF1-&”ÀZFÌ`V°¥@EU™ZUÙ’`54ä\k4¥¾ŠEvÑC‰Yà9×ÛúÑÐm^‘¶™–t“_îhˆÛT•G}AN¤uQ5]T”}ótÔÃÞ­�˜@u E!i™Y•$•ZÚA$ßßPÕD‚4DUQ4€Yê+è
èÓAMæ4ûXs‡	Ðôu¦‹‚d�4Ñu¨ášÍtÛŠ5íÒàµ4ïCQoƒˆùPmtï€€(î^Ú�÷Ž#è9(Y[¸F�mÜ~€tEiu§u:YÞúZ[5_MEQauš¤e`5* Z
—eÕ â	™VšÙa’A‚B¥J¨âz¹’D¤4k}¦Ê´~ýÄüt?EP�ÑÚÆ’ìp›
^©TU €t� TY$vY5ÑÙÕåE§UuiÕZÕj›äšcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'*fSDì©!|´løÏosŠ¡ûÙk‘9>Åì¶8íû÷ï?èßÓ>øÏ¯FþçB.àSÄ4Â@øÐçÇàQÓA ããD·ôß4ãõOà6õ÷ôã½Ô/ÓqÇ@ôô‰!CÀÑ†=?Óø?€ýÑ_L <y>ÜA¸TSÔ3ôÑ£OÓ‡ßûôïZüƒ/Në]ðÑ·è=ÃÑ tSôôþÑ#¿­O¿CØß í>½û8ðOCûÜ7ý/ôOñÑ ¿ƒÝ6{DäèãøBHAŸx ©¹PE�7Ž=`8ß‹õ_ÓKíðÿ~ôáÝ?N4b×Õ~�³ô�s·Ýõë—M=ÖóÓßµ´_ßx¢ýÑ>=êäÛ×E¯´CõïÛ–Ð>:õMþ"8`5ÿC|8AF4Ýï–‡P›^ôçß}7ë½Ns¶ÿNô?ôûF­‹Ó¿ŽÚ½?]þH"ÓFÔF.N=÷?}BçHB÷­ÞSÿm µÐpô>¨ô3Ð}=ÿc‘Çj€Cü;ý?ý*=ÿÃÝÝ–/Í?Ø Ta€4Å?´Dsþ€­ãýùÞú/tÿ¸:øBï¿¶k‹âoÓ¼Ôºí¸P=+ãÓQÀÚàA,`üð<Õ½Šž¬�ýBñoG£�¸'GLE0ÃDÐ	�úÍàÄ>õëÓA=7ì‡@ôÿ0Ã‚}ï�µ-ÿ6ô=Oý_×Ñ~ïû€P„îsóÏKp`Ef-±.µ=ÜôNÎ?mùö/ãëï�Yÿysÿ úñ ÛñOÓðÏ�¿¹ö~>pã?Dýõ4óÙôã?ìî¢û·æÔãÔ.ßQ8û?=OÓ¾�ø#õøý@ïïnøþ>Ãû3¾ßîwïü=O�?xòûéÃvïü‹¿½ »üOSCÿCûë¹ü<Ä;B SëëŽµ;gŸ†˜×¦úa‰øiŽNo¦¿GœÙ¿ŒfôÌ<Åã;Â?- 4LÝ³÷KüE( h\ùBÀ%xHbÈžóê¯Ä EòfR4’Ï!rº«5À¨óÑwNëÍ»¾·3óÏçá=½Ï
Ì®<ûóâûáxËÏ.BR¥NÐ±ê/ïèçä{Oïs¨øŽŽ×¹Ë¨«*¸®3ÊtoSˆ|3ç9÷Ø1ÄL>?=�<Û|7ø\2¬N0DÿÔÐ<Ø}uQ7Õþ­?çã„÷!€ç¹÷¬7çý0çëãsÛ]pÃ�õßÍ±œAÃŽ7!:ÓŸ9Ä<<Èl1ëP‚|:ÿtÈn·ÇQ¶óMsß Møßör8 4ë?7ëOŒÇ9çN²=ØÞµ=½ÿM¾4\ ÿb³ëÏ2�Ó]ñ8²ŸMÏ­GÿlúÏFüÎÒËÖ¸òóÁü;ßìÄRë—croÃù‡Ä�OÜ#>Ï1Ç/ô'7û<ó+<yÿ=Ì÷|±¼=Ûß�Eî¾ÜÌ°;°Ô"ÌpÏ0ƒ�÷<2Å0¬ñÌØ<J
ü8<Í5Ñ�Cïƒ<ßÔó ¯ƒß,2ìü}ô@¸3Ë#¿Á C3À#óëàGï3ü×ïúçÃ­òß<rÄ#ñAó7à�‡óSð�…m~àiÕj!Q¡àšéyŠ;êœæ g8êXú‰¶9£¯Éƒ«j»ï¢eo‚Ž.êa¢Ê-r‚#xŠŽ×ÿx(h÷ß‹Nú¼«:¿ûù£Ü0^Û<øú?8àt°â€ÞkÈ"‹ S‡/«Ø8ìøŠ=øã0¾¸ç/ëíoL¯¯Ì>8ðî0û¯2ï>³ûGÁ»úƒÏ¿Ï‚~¥Xzu8‘ºOz©TU �@å­ï×eŽª:'€»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒI+bÛ¦šÒjeèÕ…›DHD¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸;“›óþ^óô¯?k¼ÿ`áåªªjcD<CÏ°û„æ½’Cª Cäz£DpýZÉm‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷áË”WÚi¶¥ù•Y›qêË³2¬!‡Uû‡v4ïÁ-Ó¢âS<ÑÔü´ËL À‹ àéyŒ6Ñ4hž#}˜Â!Yótc%UŸ6ÐÏk=­3^(Àû¬æœôàÅ@#�CR€—=«ô·¢UÇHõ8           •  —  ñ  	   è  I  N  ]  d  f  +W  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  .¿<?xml version="1.0"?><Features nextValidID="25"><Feature recentID="24" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10423" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9632-10423" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10424-10492" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="7191" maxFusedRunOn="7191"><Segment range="5806-6468" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6592-7191" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="8873-9461" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="20" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7192-7779" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5479-5747" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1076-1309" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7853-8086" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5255-5466" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2011-2214" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-414" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8298-8433" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="458-583" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8158-8279" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1804-1921" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10493-10597" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6588" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="7191" maxFusedRunOn="7191" detectionMode="exactProteinMatch"><Segment range="6538-6591" color="#cc99b2" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="8519-8525" color="#ffffff" type="standard"/><Segment range="8526-8543" color="#ffffff" type="standard"/><Segment name="-35" range="8544-8549" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="8564-8585" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="8495-8511" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7662" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7279" maxFusedRunOn="7279" visible="0" detectionMode="exactProteinMatch"><Segment range="7663-7674" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="SYNPO" directionality="1" translationMW="99466.71" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5193" maxFusedRunOn="5193"><Segment range="2311-5097" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5193" maxFusedRunOn="5193" detectionMode="exactProteinMatch"><Segment range="5164-5190" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5193" maxFusedRunOn="5193" detectionMode="exactProteinMatch"><Segment range="5134-5160" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5193" maxFusedRunOn="5193" detectionMode="exactProteinMatch"><Segment range="5104-5130" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  3<?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="R1" sequence="GCTGGATATCGCAGggatccCTATGCGTAGTCTGGGAC" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5175-5212" boundStrand="1" annealedBases="GCTGGATATCGCAGggatccCTATGCGTAGTCTGGGAC" meltingTemperature="70"><Component hybridizedRange="5175-5212" bases="GCTGGATATCGCAGggatccCTATGCGTAGTCTGGGAC"/></BindingSite><BindingSite simplified="1" location="5175-5212" boundStrand="1" annealedBases="GCTGGATATCGCAGggatccCTATGCGTAGTCTGGGAC" meltingTemperature="70"><Component hybridizedRange="5175-5212" bases="GCTGGATATCGCAGggatccCTATGCGTAGTCTGGGAC"/></BindingSite></Primer><Primer recentID="1" name="F1" sequence="TACCCATACGACGTACCTGA" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5103-5122" boundStrand="0" annealedBases="TACCCATACGACGTACCTGA" meltingTemperature="55"><Component hybridizedRange="5103-5122" bases="TACCCATACGACGTACCTGA"/></BindingSite><BindingSite simplified="1" location="5103-5122" boundStrand="0" annealedBases="TACCCATACGACGTACCTGA" meltingTemperature="55"><Component hybridizedRange="5103-5122" bases="TACCCATACGACGTACCTGA"/></BindingSite></Primer></Primers>
  ?<Notes>
<UUID>cf3c1581-de24-489b-8601-ed8aa81d943f</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.6.7</Created>
<LastModified UTC="5:29:26">2022.3.30</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  c<StrandColors><TopStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="2310..2709" colors="red"/><ColorRange range="5193..11095" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="2310..2709" colors="red"/><ColorRange range="5193..11095" colors="black"/></BottomStrand></StrandColors>  =ÿ        +U        +Us"K¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆu•À»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒü]øTÅ@WÑ;»Ð{ŽÌÜ5Äx!'=UªLäPDF1-&”ÀZFÌ`V°¥@EU™ZUÙ’`54ä\k4¥¾ŠEvÑC‰Yà9×ÛúÑÐm^‘¶™–t“_îhˆÛT•G}AN¤uQ5]T”}ótÔÃÞ­�˜@u E!i™Y•$•ZÚA$ßßPÕD‚4DUQ4€Yê+è
èÓAMæ4ûXs‡	Ðôu¦‹‚d�4Ñu¨ášÍtÛŠ5íÒàµ4ïCQoƒˆùPmtï€€(î^Ú�÷Ž#è9(Y[¸F�mÜ~€tEiu§u:YÞúZ[5_MEQauš¤e`5* Z
—eÕ â	™VšÙa’A‚B¥J¨âz¹’D¤4k}¦Ê´~ýÄüt?EP�ÑÚÆ’ìp›
^©TU €t� TY$vY5ÑÙÕåE§UuiÕZÕj›äšcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'*fSDì©!|´løÏosŠ¡ûÙk‘9>Åì¶8íû÷ï?èßÓ>øÏ¯FþçB.àSÄ4Â@øÐçÇàQÓA ããD·ôß4ãõOà6õ÷ôã½Ô/ÓqÇ@ôô‰!CÀÑ†=?Óø?€ýÑ_L <y>ÜA¸TSÔ3ôÑ£OÓ‡ßûôïZüƒ/Në]ðÑ·è=ÃÑ tSôôþÑ#¿­O¿CØß í>½û8ðOCûÜ7ý/ôOñÑ ¿ƒÝ6{DäèãøBHAŸx ©¹PE�7Ž=`8ß‹õ_ÓKíðÿ~ôáÝ?N4b×Õ~�³ô�s·Ýõë—M=ÖóÓßµ´_ßx¢ýÑ>=êäÛ×E¯´CõïÛ–Ð>:õMþ"8`5ÿC|8AF4Ýï–‡P›^ôçß}7ë½Ns¶ÿNô?ôûF­‹Ó¿ŽÚ½?]þH"ÓFÔF.N=÷?}BçHB÷­ÞSÿm µÐpô>¨ô3Ð}=ÿc‘Çj€Cü;ý?ý*=ÿÃÝÝ–/Í?Ø Ta€4Å?´Dsþ€­ãýùÞú/tÿ¸:øBï¿¶k‹âoÓ¼Ôºí¸P=+ãÓQÀÚàA,`üð<Õ½Šž¬�ýBñoG£�¸'GLE0ÃDÐ	�úÍàÄ>õëÓA=7ì‡@ôÿ0Ã‚}ï�µ-ÿ6ô=Oý_×Ñ~ïû€P„îsóÏKp`Ef-±.µ=ÜôNÎ?mùö/ãëï�Yÿysÿ úñ ÛñOÓðÏ�¿¹ö~>pã?Dýõ4óÙôã?ìî¢û·æÔãÔ.ßQ8û?=OÓ¾�ø#õøý@ïïnøþ>Ãû3¾ßîwïü=O�?xòûéÃvïü‹¿½ »üOSCÿCûë¹ü<Ä;B SëëŽµ;gŸ†˜×¦úa‰øiŽNo¦¿GœÐoã½31A¸ÆÎð�ËH7lýÒÿJ ÃZ>DPƒ0	^R²'ƒ¼ú«ñ |™”�$³È\®ªÍp*<ô]Ó…:Ä³nï­ÌüóùøCÏosÂ„³+�>üø‚>ø^2óË�”©S´,GÀú‹Ã;úùùÓÃ»Üê>ã£µîrê*Ê®+Œò�ÔâùÎG=öLq�Ï`FÄ�6ßþ�+‚õ46]FMõC?‡ëOùøá=È`9Ã®}ëùÿL9Ä_úøÜö×\0ç}wólE'pÃ#�ÈN´çÎq2zÔ €ß¿Ä2­ñÔm¼Ó\÷È~7ý€Æ\Ž:ÏÍúÓã1ÃyÓ¬‚Ov7­DÏoÓo� ?Ø¬úóÌ�ãt×|Nl�'ÓsëQÿÛ>³Ñ¿3´²õ®<�Ã|ð÷Á»1DºåØÂ�[ðþañ'S÷Ï³ßÌqËýÉÍþÏ<ÊÏÏs=ß,oÏvÄÀ·ÄãQ{¯·3,Çl5€³3Ì Âà}ÏL±L Â+<FÃó6„Â¿3Mt`PûàÏ7ÃõüÈ+à÷Ë»?}P.LòÈïðHÃÄÌðüúøûÌÿ5ûþ¹ÄÃ°ë|·Ï±üP|Íø#aüÔü#á[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢mŽhëò`êÚ®ûè™[à£‹º˜A(ƒò‹C\ƒ ˆÞ"£‡5ÿÞ
 Ú=÷âÓ¾¯êÎ¯þþh÷¶Ï>>�Î8 ì8 7‡Zò¢ÀáËêö;>"�~¸Ì/®9ËúÃû[ÓëëóŽ<;Œ>ëÌ»Ï¬þÑÄ°nþ óïóàŸ©GÖ�CN$n“ÞªU@àP9k{õÅYFãªŽ‰à.î
GÑ¸O€Nî^@_Þ:SíeN�¡#­I"#òâˆ‡¸%äFþÑþ©-"[¹4’JØ¶é¦´š™z5EafÑè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.äæüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóì>á9¯E$„ê¨ù¨Ñ?V²[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=øråöšm‡i~eVC&ÜCú²ìÌ«aÂ~áÝ�;ðGtè¸”Á�4u?-2Ó(0"À8:^c´M'„Hßf0ˆV|ÝÉAgÍ´3ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ=N            •  —  ñ  	   è  F  K  Z  a  c  +T  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  .¿<?xml version="1.0"?><Features nextValidID="25"><Feature recentID="24" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5310" maxFusedRunOn="5310" detectionMode="exactProteinMatch"><Segment range="5104-5130" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5310" maxFusedRunOn="5310" detectionMode="exactProteinMatch"><Segment range="5134-5160" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5310" maxFusedRunOn="5310" detectionMode="exactProteinMatch"><Segment range="5164-5190" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="21" name="SYNPO" directionality="1" translationMW="99466.71" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5310" maxFusedRunOn="5310"><Segment range="2311-5097" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7659" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7276" maxFusedRunOn="7276" visible="0" detectionMode="exactProteinMatch"><Segment range="7660-7671" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="8492-8508" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="8561-8582" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="8516-8522" color="#ffffff" type="standard"/><Segment range="8523-8540" color="#ffffff" type="standard"/><Segment name="-35" range="8541-8546" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6585" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="7188" maxFusedRunOn="7188" detectionMode="exactProteinMatch"><Segment range="6535-6588" color="#cc99b2" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10490-10594" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1804-1921" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8155-8276" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="458-583" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8295-8430" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-414" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2011-2214" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5252-5463" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7850-8083" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1076-1309" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5476-5744" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7189-7776" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="8870-9458" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="2" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6589-7188" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="7188" maxFusedRunOn="7188"><Segment range="5803-6465" color="#05fd14" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10420" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9629-10420" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10421-10489" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  =<Notes>
<UUID>b64490df-681d-4c2f-a3de-086749b86fba</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.6.7</Created>
<LastModified UTC="5:3:9">2022.3.30</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	<StrandColors><TopStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="5190..11092" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="5190..11092" colors="black"/></BottomStrand></StrandColors>  7ì     
  *þ        *þs"K¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆu•À»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒü]øTÅ@WÑ;»Ð{ŽÌÜ5Äx!'=UªLäPDF1-&”ÀZFÌ`V°¥@EU™ZUÙ’`54ä\k4¥¾ŠEvÑC‰Yà9×ÛúÑÐm^‘¶™–t“_îhˆÛT•G}AN¤uQ5]T”}ótÔÃÞ­�˜@u E!i™Y•$•ZÚA$ßßPÕD‚4DUQ4€Yê+è
èÓAMæ4ûXs‡	Ðôu¦‹‚d�4Ñu¨ášÍtÛŠ5íÒàµ4ïCQoƒˆùPmtï€€(î^Ú�÷Ž#è9(Y[¸F�mÜ~€tEiu§u:YÞúZ[5_MEQauš¤e`5* Z
—eÕ â	™VšÙa’A‚B¥J¨âz¹’D¤4k}¦Ê´~ýÄüt?EP�ÑÚÆ’ìp›
^©TU €t� TY$vY5ÑÙÕåE§UuiÕZÕj›äšcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'*fSDì©!|´løÏosŠ¡ûÙk‘9>Åì¶8íû÷ï?èßÓ>øÏ¯FþçB.àSÄ4Â@øÐçÇàQÓA ããD·ôß4ãõOà6õ÷ôã½Ô/ÓqÇ@ôô‰!CÀÑ†=?Óø?€ýÑ_L <y>ÜA¸TSÔ3ôÑ£OÓ‡ßûôïZüƒ/Në]ðÑ·è=ÃÑ tSôôþÑ#¿­O¿CØß í>½û8ðOCûÜ7ý/ôOñÑ ¿ƒÝ6{DäèãøBHAŸx ©¹PE�7Ž=`8ß‹õ_ÓKíðÿ~ôáÝ?N4b×Õ~�³ô�s·Ýõë—M=ÖóÓßµ´_ßx¢ýÑ>=êäÛ×E¯´CõïÛ–Ð>:õMþ"8`5ÿC|8AF4Ýï–‡P›^ôçß}7ë½Ns¶ÿNô?ôûF­‹Ó¿ŽÚ½?]þH"ÓFÔF.N=÷?}BçHB÷­ÞSÿm µÐpô>¨ô3Ð}=ÿc‘Çj€Cü;ý?ý*=ÿÃÝÝ–/Í?Ø Ta€4Å?´Dsþ€­ãýùÞú/tÿ¸:øBï¿¶k‹âoÓ¼Ôºí¸P=+ãÓQÀÚàA,`üð<Õ½Šž¬�ýBñoG£�¸'GLE0ÃDÐ	�úÍàÄ>õëÓA=7ì‡@ôÿ0Ã‚}ï�µ-ÿ6ô=Oý_×Ñ~ïû€P„îsóÏKp`Ef-±.µ=ÜôNÎ?mùö/ãëï�Yÿysÿ úñ ÛñOÓðÏ�¿¹ö~>pã?Dýõ4óÙôã?ìî¢û·æÔãÔ.ßQ8û?=OÓ¾�ø#õøý@ïïnøþ>Ãû3¾ßîwïü=O�?xòûéÃvïü‹¿½ »üOSCÿCûë¹ü<Ä;B SëëŽµ;øÆoLÃÌPn1³¼#òÒDÍÛ?t¿ÄR€ °Ö…À�‘ ÌW€T†,‰àï>ªü@€_&e#I,ò+ª³\
�=táN± ìÛ»ës?<þ~óÛÜð¡,ÊãÏ¿> �¾Œ¼òä%*Tíð>¢ðÎþ�>~G´ðî÷:�‡øèí{œºŠ²«Šã<§Fõ8‡Ã>s‘Ï}�DÃãóØ±#Í·Ã…Ã JÄà€Ã@ßýMÍ‡×Q…}PÏáúÓþ~8OrpëŸzÃ~Óqþ¾7=µ×9ß]üÛIÄ0Èãr­9óœCÃÌ†Ãµ 7Ã¯ñL†ë|uo4×=òß�ÿ`1—#‚N³ó~´øÌpÃžtë �ÓÝ�ëQ3ÛßôÛãEÀö+>¼ó A8Ý5ß€Û IôÜúÔöÏ¬ôoÌí,½k� pß<Ã½ðnÌ@Ñ.¹v0‡ Aü?˜|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øV×î�V¢	®—˜£¾©ÎbsŽ¥�¨›cš:ü˜:¶«¾ú&Vø(âî¦J ü¢Ð× È"7ˆ¨áÍ÷‚€6�}ø´ï«Àz³«ÿ¿š=Ãí³Ï�£óŽ@;(áÖ¼‚(°8rú½ƒŽÏˆ£ß�®3ËëŽrþ°þÖôÁúúüÃã�ãºó.óë?´q,¿¨<ûüø'êQõ‡§PÓ‰¤÷ª•EP xZÞýqVQ¸ê£¢x»‚‘ônà»ƒ—�÷Ž”ûYS£èHëRHˆü¸¢!î	y¿´ªKH�VîM$’¶-ºi­&¦^�QXY´D„Az*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹ƒ¹9¿?åï?Jóö»ÏöZª¦¦4CÄ<û¸NkÑI!:ª>Gª4GÕ¬–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~¹E}¦›aÚ_™U�É·þ¬»3*Âp…_¸wcNüÂÝ:.%0cÀMOË@Ì´ÀŠ°—˜ÃmF‰á7ÙŒ"Ÿ7F2PEYómö³ÚÐã5â€P1°ŒºÎiÏNT b8Ô1¥(	sÐ*¿KqÊ%\pô�S           •  —  ñ  	   è  ï  ô    
    *ý  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  (Â<?xml version="1.0"?><Features nextValidID="22"><Feature recentID="21" name="SYNPO" directionality="1" translationMW="99466.71" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5223" maxFusedRunOn="5223"><Segment range="2311-5097" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7572" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7189" maxFusedRunOn="7189" visible="0" detectionMode="exactProteinMatch"><Segment range="7573-7584" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="8405-8421" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="8474-8495" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="8429-8435" color="#ffffff" type="standard"/><Segment range="8436-8453" color="#ffffff" type="standard"/><Segment name="-35" range="8454-8459" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6498" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="7101" maxFusedRunOn="7101" detectionMode="exactProteinMatch"><Segment range="6448-6501" color="#cc99b2" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10403-10507" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1804-1921" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8068-8189" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="458-583" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8208-8343" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-414" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2011-2214" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5165-5376" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7763-7996" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1076-1309" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5389-5657" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7102-7689" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="8783-9371" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="2" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6502-7101" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="7101" maxFusedRunOn="7101"><Segment range="5716-6378" color="#05fd14" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10333" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9542-10333" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10334-10402" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  =<Notes>
<UUID>b64490df-681d-4c2f-a3de-086749b86fba</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.6.7</Created>
<LastModified UTC="5:3:1">2022.3.30</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	<StrandColors><TopStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="5103..11005" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="5103..11005" colors="black"/></BottomStrand></StrandColors>  7Ê        *ø        *øs"K¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆu•À»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒü]øTÅ@WÑ;»Ð{ŽÌÜ5Äx!'=UªLäPDF1-&”ÀZFÌ`V°¥@EU™ZUÙ’`54ä\k4¥¾ŠEvÑC‰Yà9×ÛúÑÐm^‘¶™–t“_îhˆÛT•G}AN¤uQ5]T”}ótÔÃÞ­�˜@u E!i™Y•$•ZÚA$ßßPÕD‚4DUQ4€Yê+è
èÓAMæ4ûXs‡	Ðôu¦‹‚d�4Ñu¨ášÍtÛŠ5íÒàµ4ïCQoƒˆùPmtï€€(î^Ú�÷Ž#è9(Y[¸F�mÜ~€tEiu§u:YÞúZ[5_MEQauš¤e`5* Z
—eÕ â	™VšÙa’A‚B¥J¨âz¹’D¤4k}¦Ê´~ýÄüt?EP�ÑÚÆ’ìp›
^©TU €t� TY$vY5ÑÙÕåE§UuiÕZÕj›äšcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'*fSDì©!|´løÏosŠ¡ûÙk‘9>Åì¶8íû÷ï?èßÓ>øÏ¯FþçB.àSÄ4Â@øÐçÇàQÓA ããD·ôß4ãõOà6õ÷ôã½Ô/ÓqÇ@ôô‰!CÀÑ†=?Óø?€ýÑ_L <y>ÜA¸TSÔ3ôÑ£OÓ‡ßûôïZüƒ/Në]ðÑ·è=ÃÑ tSôôþÑ#¿­O¿CØß í>½û8ðOCûÜ7ý/ôOñÑ ¿ƒÝ6{DäèãøBHAŸx ©¹PE�7Ž=`8ß‹õ_ÓKíðÿ~ôáÝ?N4b×Õ~�³ô�s·Ýõë—M=ÖóÓßµ´_ßx¢ýÑ>=êäÛ×E¯´CõïÛ–Ð>:õMþ"8`5ÿC|8AF4Ýï–‡P›^ôçß}7ë½Ns¶ÿNô?ôûF­‹Ó¿ŽÚ½?]þH"ÓFÔF.N=÷?}BçHB÷­ÞSÿm µÐpô>¨ô3Ð}=ÿc‘Çj€Cü;ý?ý*=ÿÃÝÝ–/Í?Ø Ta€4Å?´Dsþ€­ãýùÞú/tÿ¸:øBï¿¶k‹âoÓ¼Ôºí¸P=+ãÓQÀÚàA,`üð<Õ½Šž¬�ýBñoG£�¸'GLE0ÃDÐ	�úÍàÄ>õëÓA=7ì‡@ôÿ0Ã‚}ï�µ-ÿ6ô=Oý_×Ñ~ïû€P„îsóÏKp`Ef-±.µ=ÜôNÎ?mùö/ãëï�Yÿysÿ úñ ÛñOÓðÏ�¿¹ö~>pã?Dýõ4óÙôã?ìî¢û·æÔãÔ.ßQ8û?=OÓ¾�ø#õøý@ïïnøþ>Ãû3¾ßîwïü=O�?xòûéÃvïü‹¿½ »üOSCÿCûë¹ü<Ä;B SëëŽµ¿ŒfôÌ<Åã;Â?- 4LÝ³÷KüE( h\ùBÀ%xHbÈžóê¯Ä EòfR4’Ï!rº«5À¨óÑwNëÍ»¾·3óÏçá=½Ï
Ì®<ûóâûáxËÏ.BR¥NÐ±ê/ïèçä{Oïs¨øŽŽ×¹Ë¨«*¸®3ÊtoSˆ|3ç9÷Ø1ÄL>?=�<Û|7ø\2¬N0DÿÔÐ<Ø}uQ7Õþ­?çã„÷!€ç¹÷¬7çý0çëãsÛ]pÃ�õßÍ±œAÃŽ7!:ÓŸ9Ä<<Èl1ëP‚|:ÿtÈn·ÇQ¶óMsß Møßör8 4ë?7ëOŒÇ9çN²=ØÞµ=½ÿM¾4\ ÿb³ëÏ2�Ó]ñ8²ŸMÏ­GÿlúÏFüÎÒËÖ¸òóÁü;ßìÄRë—croÃù‡Ä�OÜ#>Ï1Ç/ô'7û<ó+<yÿ=Ì÷|±¼=Ûß�Eî¾ÜÌ°;°Ô"ÌpÏ0ƒ�÷<2Å0¬ñÌØ<J
ü8<Í5Ñ�Cïƒ<ßÔó ¯ƒß,2ìü}ô@¸3Ë#¿Á C3À#óëàGï3ü×ïúçÃ­òß<rÄ#ñAó7à�‡óSð�…m~àiÕj!Q¡àšéyŠ;êœæ g8êXú‰¶9£¯Éƒ«j»ï¢eo‚Ž.êa¢Ê-r‚#xŠŽ×ÿx(h÷ß‹Nú¼«:¿ûù£Ü0^Û<øú?8àt°â€ÞkÈ"‹ S‡/«Ø8ìøŠ=øã0¾¸ç/ëíoL¯¯Ì>8ðî0û¯2ï>³ûGÁ»úƒÏ¿Ï‚~¥Xzu8‘ºOz©TU �@å­ï×eŽª:'€»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒI+bÛ¦šÒjeèÕ…›DHD¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸;“›óþ^óô¯?k¼ÿ`áåªªjcD<CÏ°û„æ½’Cª Cäz£DpýZÉm‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷áË”WÚi¶¥ù•Y›qêË³2¬!‡Uû‡v4ïÁ-Ó¢âS<ÑÔü´ËL À‹ àéyŒ6Ñ4hž#}˜Â!Yótc%UŸ6ÐÏk=­3^(Àû¬æœôàÅ@#�CR€—=«ô·¢UÇHõ8           •  —  ñ  	   î  ý      *÷  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  (©<?xml version="1.0"?><Features nextValidID="22"><Feature recentID="21" name="SYNPO" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5217" maxFusedRunOn="5217"><Segment range="2311-5097" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7566" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="7183" maxFusedRunOn="7183" visible="0" detectionMode="exactProteinMatch"><Segment range="7567-7578" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="8399-8415" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="8468-8489" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="8423-8429" color="#ffffff" type="standard"/><Segment range="8430-8447" color="#ffffff" type="standard"/><Segment name="-35" range="8448-8453" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6492" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="7095" maxFusedRunOn="7095" detectionMode="exactProteinMatch"><Segment range="6442-6495" color="#cc99b2" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10397-10501" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1804-1921" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8062-8183" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="458-583" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8202-8337" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-414" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2011-2214" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5159-5370" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7757-7990" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1076-1309" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5383-5651" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7096-7683" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="8777-9365" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="2" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6496-7095" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="7095" maxFusedRunOn="7095"><Segment range="5710-6372" color="#05fd14" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10327" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9536-10327" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10328-10396" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>b64490df-681d-4c2f-a3de-086749b86fba</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.6.7</Created>
<LastModified UTC="5:2:24">2022.3.30</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	<StrandColors><TopStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="5097..10999" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="5097..10999" colors="black"/></BottomStrand></StrandColors>  4(     R            s"K¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆu•À»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒü]øTÅ@WÑ;»Ð{ŽÌÜ5Äx!'=UªLäPDF1-&”ÀZFÌ`V°¥@EU™ZUÙ’`54ä\k4¥¾ŠEvÑC‰Yà9×ÛúÑÐm^‘¶™–t“_îhˆÛT•G}AN¤uQ5]T”}ótÔÃÞ­�˜@u E!i™Y•$•ZÚA$ßßPÕD‚4DUQ4€Yê+è
èÓAMæ4ûXs‡	Ðôu¦‹‚d�4Ñu¨ášÍtÛŠ5íÒàµ4ïCQoƒˆùPmtï€€(î^Ú�÷Ž#è9(Y[¸F�mÜ~€tEiu§u:YÞúZ[5_MEQauš¤e`5* Z
—eÕ â	™VšÙa’A‚B¥J¨âz¹’D¤4k}¦Ê´~ýÄüt?EP�ÑÚÆ’ìp›
^©TU €t� TY$vY5ÑÙÕåE§UuiÕZÕj›äšcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'*fSDì©!|´løÏosŠ¡ûÙk‘9>Åì²¤[þ1›Ó0óŒlïü´€Ñ3vÏÝ/ñ  @,5¡p#äE3 •à!‹"x;Ïª¿ É™HÒK<…Êê¬×£ÏEÝ8S¬@;6îúÜÏÏ?Ÿ„<ö÷<(K2¸óïÏˆ#ï…ã/<¹	J•;BÄ|¨¼3¿ OŸ‘í<;½Î£áþ:;^ç.¢¬ªâ¸Ï)Ñ½N!ðÏœäsß`DÇ0øüölHómðßápÈ±8 0Á7ÿS@óaõÔaDßT3ø~´ÿŸŽÜ†œ:çÞ°ßŸôÃœEÿ¯�ÏmuÃw×6ÄRq28Ü„ëN|çðó!°Ç­BðëüAÓ!ºßFÛÍ5Ï|�7ãØeÈà€Ó¬üß­>30ç�:È t÷czÔLö÷ý6øÑpýŠÏ¯<ÈN7MwÄà6È}7>µý³ë=ó;K/ZãÈ7Ïðï|³4AK®]Œ!ÈE¿æu?pŒû=üÇ¿Ð<œßìóÌ¬ñçü÷3ÝòÆð|÷lL|N5ºûs2ÀìvÃPˆ0qÃ<Â.ÜðDËÀ"³Äl?3`ñ(L+ðàó4×F¾ó|?POÌ‚¾|°Ë³ñ÷Ð5à4Ï,Žÿ�<LÏ �Ï¯�¼Ïó_¿ëœL;·Ë|ñË�ÅÌß‚6ÍOÂ>µû�§U¨…F‡‚k¥æ(ïªs˜�œã©cê&ØæŽ¿&­ªï¾‰•¾
8»©„ˆ?(´5È2�â*8s_ýà £ß~-;êð¬êÿïæ�pÁ{lóãèüã�ÐÃŠxu¯ Š,N¾¯`ã³â(÷àkŒÀrúãœ¿¬?µ½0~¾¿0øãÃ¸Ãî¼Ë¼úÏíKïê>ÿ>	ú”}aéÔ4âFé=ê¥QT –·¿\U”n:¨èžîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“I$­‹nškI©—£TVm!^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàîNoÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ÃîšôRHAª�‘ê�Ãõk%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡.Q_i¦Øv—æUd2mÄ?«.ÌÊ°†!WîØÓ¿p·N‹‰LðGSòÐ3-0"ƒ,ƒ¥æ0ÛDÑ¢xD�öc…gÍÑŒ”V|ÛC=¬ö´8Íx l#î³šsÓƒ Ž5iJ\ô
¯ÒÜr‰W=#Ô8           •  —  ñ  	   	
  	  	  	$  	+  	-     !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ']<?xml version="1.0"?><Features nextValidID="21"><Feature recentID="20" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4789" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4406" maxFusedRunOn="4406" visible="0" detectionMode="exactProteinMatch"><Segment range="4790-4801" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="5622-5638" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="5691-5712" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="5646-5652" color="#ffffff" type="standard"/><Segment range="5653-5670" color="#ffffff" type="standard"/><Segment name="-35" range="5671-5676" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3715" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="4318" maxFusedRunOn="4318" detectionMode="exactProteinMatch"><Segment range="3665-3718" color="#cc99b2" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7620-7724" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1804-1921" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5285-5406" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="458-583" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5425-5560" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-414" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2011-2214" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2382-2593" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4980-5213" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1076-1309" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2606-2874" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4319-4906" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6000-6588" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="2" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3719-4318" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="4318" maxFusedRunOn="4318"><Segment range="2933-3595" color="#05fd14" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7550" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6759-7550" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7551-7619" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>b64490df-681d-4c2f-a3de-086749b86fba</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.6.7</Created>
<LastModified UTC="5:1:32">2022.3.30</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  a<StrandColors><TopStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="2310..2319" colors="red"/><ColorRange range="2320..8222" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2309" colors="black"/><ColorRange range="2310..2319" colors="red"/><ColorRange range="2320..8222" colors="black"/></BottomStrand></StrandColors>   ¤ý7zXZ  æÖ´F !   t/å£ãÀJ b] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷ÀnãýJ„<Ú+(ný W¨”ÂŒ’	”Vw8ýös‡êœöÝ2ÍÖu:`ZQ±ÒQþAXË�¿º™Ï_“&žâã)�Ìñ»íõØã&)NßÛ@õqn‡ÙÊ›:uÀyç \î0¶¨l(™ìqN®VÓ�óWLKJ{—Dø~(ôD¢ÓNÏ	FÆ
aa±`œ+'ào�ÖË@C>õÊ¼k[¨¡VMø¢Ùä##Ø„êÎ¦<!ãáü}·"iP+4§ª–Öô“¼ÿæŒ/íÈ5
†õ-8…o>Êëq
ßØæ–ïýönØý¸ô<	÷�M„Úy<jîoRÆ‘�µ%)ÕN_��ð\ó»#obf<¾kÍÚœ‡½ž6b:¹ù‚ÿcÇ•?GÝÉž`+¿y	ì\TÃ®å„LÈƒj�GÿC´©"µùu-¨*8ã©÷9{—Ñš�É¦MÈM
D}ç/´öS1�ígôJe¨ˆ¸b&¨‰)8æÒ¦ø™“bÑ�uL!*™5ë?ßD£˜ÈÙ]´nH}èÏá+ñ3ÕŠÿüpr£è)=—³ä}­žu£ž-6Ð	ã~ÓÏaÔØÄµ0Kn6‡C3z‚UÞ…xÑÝüæî'1zEÝnÏ‚le¸ÉYË÷žX/3O€Ž&ÿPûÐ{Ñi@bŸ=V[A­#Œz’…of$G•n“‚5ý®¡öHØK),ôåÙøáðc'{˜öî —9Wfg‹’OÍÁÅ`ú“«ù£•W›<C&Óeý¢!Ö$oÒœê‡; rlã²ëùÀc»�éä,–öÔÁîæÚF`2†ö¯:ÝÂQBádPß ÅDéˆ	—Ær®yÄ,©Öö¬v¶ç#«ž1xƒ$8¾­õ·WZ¡�b¹&o¢QSðµâcÄPmøÐúÂy*	äˆ¿ñßæ i¸
8[QKÑ¼ÿÿXˆ8E]@Ú¯(Œuíç@z•¯Gœá¯‚OE¾ìõ®EeÓ Ìzô–�î	#­þwþ±~w#[‰xøp6SÜÉuk2Ð
ÔKÉ1´r.:0iB"C#ð­àè¤VZ„(m6?B?x®›ÓxÅpŸ{ÛK•~©¢…¥¥	ÉêL�¬¾p£“¢G›p,¸O:vDö�Ž-uÜ´9©U0RUÜ–v[Š åÃ—–[›å‡>ê™ìÕ¡µ–öò=1â°#°Ïß)÷�T�ÙïW)MïÎ^­QDF3({?}õ,²Š;e½é#ÂÙ˜ºÜ*úøžáP-ónm•pù¼µ9)<ZUêySº€�züÀð¦˜Dl=êØñË)_!‰Av·w»¢ûÆÐ	œòsf7d·n$-¢N™[œ
w†R¾2ˆÍê_qÓ­|ÞŸ´éÕYdß	B0ðýzd ÌÞè—ßCöâÇ“Q«Åt¦Båª€ø5�3S6Ÿúv*Êd”àµ,#qf„8â½Q/ ~þ»q˜Yö<ÿEÕå8.ˆÆØGsZ™!§máuuÉ(p‡ñÕsL!E…õwÂW*¯1ã¢™p
°:puêo‰Ÿ÷Oaä]AI]¥ÚÕS†eñR¨káÛ%àÐ\çhëÛ¢4Å‘¬™Ö7Î%Í‚\K'`!JiH7nÔç1§>@èµý9<Sz¤:â][´ÐÕ ç\÷„& �1©lOµGQ|5×f¹—2%&á²ø‘ŠMàñêÜ/ÿ�—ˆ­ —£¥¯âËü	G×?.wª¸µÃÆt°¡ÐªrÛ7œsÛôH´”ZQ•Gÿí¡c‰;«gµ�‹Ê&z‚õî‚È B³j°œ�qnÙs)e8?Æzj@;qµ·5­GÇz‡Å™ÂµPu¥}lÖGšîÌgw¯}ÛP|~uiè98ØÉ�t¾Êcv˜‡LtÙ²-ôX„,|á8ÇãÕE¡ø#sWh´–P¡<ªÅ¥žü¥îEsÅ³,²{½%è{aÛHeËtˆñð&nXõÆë„Š\0˜)(ó¥ V¶2KÆ¨Ã6¾ÑÁè&úuø=T!áóWðû,z÷ÌÒ)ìÚ¨=/—A%
?Í'·¾øè2 Bß;Uj@&’>5!õÃ{Qˆî£�º0ý©|\8ë·[š s‰[µá'Ra±Ê«Rw’d¯{ø©G‚ÏˆõßRŠÞRîøÛó[
o©\Üöü£¨Z¥Ã^“LÆfåÃíP�j?]ó›³T—ÿ$cKTû+2\«Û¡Av0 ¤d{Æ6°¾Í�y|Ós!v³QG¯1ÁÕ7¼žÐæ˜ªÕ¡ï?ìW”„²^]šþzƒsƒì¯£T^óUa˜ƒ&”½�‰û•sf‘Û—!»~]ÅKJm¾Ò4øžÇ|­N”Ø[Ì.Žç<UÅòøD¤:äD7é¬ÕN(P5¿/2@…üÐGØ‹WàÕz’CzÍž”œýïŽE¿5T æ†FFC¡0–º#É•UÂ\~aUwQ³é9À^íŒÊ¡K±i1��:¦(æ–|/ÐµcŸCþw¦¿^XŒ<å`}c˜íÛ¸åÒ²Rú(šJeæ¬ÜÄUÈ‚Vø�ð«¼¸D\H'çß™ÁŽLOìHÎÐórR[/N5M/Ñ°ûTZl<ºBEøkÝoµ0×V}±A�ø’™u",«œdk@ÿP¸à0
 –•S^p`Ü+ù¢Q0ä^ÚÈLO4T§ÝT‹³uä‰j#‚`¯9:Jó¸2Èk¢VhPW„ÿ‚o	Ê`p}'ŽGì]òCt+ô!ÔœZY¿rc²nÌfŽ!esšÕâ-8Cz"WÒi0i“Vzù@0	Ö.ú+´ç‘xÛºbSÿ[~÷;<&XLóâUo™-Õ8E"˜ˆÔ|cG+/›�àÌD�ß†§vÄººµ)gê3Ô<Ù¼¢Ltl¬�I[$Œ*æÄH«!ïçÍ€¨3ß, ÈSó1è¯èV‹Þã¿.XötCßCý®¤`ov·xƒÃöÖÚ»Iß™¸ìèŽbš~¯³�ï‚-‹B"`�E"¤2¡q©&­ÞïàÖVnD†°’]Q
ºâˆ6äi»Þ.Ùá¨;9¦öõ!¦jJf5!Åp©Ã¾ÉÒzñ—lFº°†=+|>Í~Ñoš$u)§»Y+Û`²]3[Uï™Ðu‘zÛ|Ñtý9‘Håç(ùýd¬ß1(sù%�ô[~ ?]Ðô€C…:ô—¿E’6N…ý|³Þ·pqbâ­´FCC- RìÎôG ’@•‹EÃ»¯¼§šØ­”nÅ{8QR¶`¸­‘¶KMÔ¨2‡wÜ‘–?bF¾q%^z-°�Øâ&vz#ÔRY’eßäÞ©ª|¶ŠLæÑÝHxBæ�=ýB«ŒÑ‘±TqÈÜÛ ð¶§¿~ÏÙæS2Í×:‰×Ž ‚àâšÕ´{ˆz•ö�®Éyópõ¯ó0”@ÃFvÆZ¨ì¿Å”ÙZáž>V; lC+—î>°êØ¸ŠIPœáR¿Œ#à†%eÿú€]T¹lžÑfŽ¯cBÄ‚O¯ÉàbŒcÙ­bMmûá?`or³ÅòôîºDËeÀ°ª½`‘¶vIÎ¹ŸÃbh MÚ-saüX�+Ðêz'×ûÇ²;ø3�4ÞÆâ›a]8˜¼1æÓÇ¥»<kÿNV³o5˜qÐa cÃ¸›‰Mª'_÷�ÑÝD³AÚ}ŸlkKöÂùùÈÍrçÑÇ%:—ùdöW{¾2ê€k`j¾òùj(v?=��	X°?Þ=¥N¡Þ™Lp×¯LKº„ð·¸‡ô»ÒM·ØëÞ·lÞ”vý¾ÃÅ£Î(|)ñdQ©¬Ï“C¾—?Š!¤{¼ypG5'ëììç§
Ú‡÷uö÷4yt#ði#œ±<	¿Qä,èÔÕ÷ò�ŠªLâ¢÷À&¥Üè½É«d›±Nêoß› �:SÏâ²TÞ¹ü÷ŠŠ'Ÿ»MŒÜN“ýjŒi	ý¥‹z2oŒd¶î­ô½˜p­À¬1Nq<@&V²3=€A8ž7ðÉÔWð_—V»6–"xØ¥�Â±¬ÞÊÕ\¹ÞIÄb>b}ˆr�F÷ø™nÇ<AîjÇDõIêõ¡ž!.»˜µQ6–�>Ý¸ÝóËøƒc ‹žZÅíJ3UNBÄi¹3(_BQM×¦»«Á58�²CI-—Ìï ^V6L2²á’Ž„Kq8ÀJ+nhËš<Ó„
�Ñý>ÝÂ2ûoa½øQ¾¥’£íe>†Àóú�>õ ÷{�´ØFY×ª:%íæãD²,¡��Ü³ÆRù*þƒ.w¨þVÖ¤MDiòhfe€“#{|�Êö¤¹î¾‘%×£m0áP™Ê4ž§îîË®|^úFhsøÞ›ü[z.Û™âzH|�­z�GŽ4¾E§3Ôg2VØýÐCÿ‰_î'*Û!a.”&Ó¡¡L7GÞî‚Ä”‘Çb	<ÆÌ‰>EºËOùªÎññxEhñdÂö3!†ÃùÃ:Ÿ NÀ?Dzˆ¼¤¾ÂÇîˆµ3x;Q›w9 ˜ÀF|ÔC’bæÝ�&ØîöiÚ¯jƒ�°ttÆ$‰Ö®ÙÆ"¨ÛËt¸û ªøM7OÙÑ±}ýîÕF5Ã†äÆôë÷UG½_þ} ¸f˜�ÚzGHMÂ¿7`¸ìvpFf´§rOØü=&J…k>_<‹íªdÒ)Ž]�ÿï%ñŸ/.æÿV©ñ­q\÷àr—U3kdOÚ}²QHE6*ÄiÞûu^O!GK™“âæôDƒŽ\¡ˆ“B¯Ò•ü
((�ÿ×Ø	�+¹‰ÿæãczãë	¨ˆ±¹Šõ„èÜ!°-äER-rH ¤�ÂÉ¾†ÿúÿ× BôYbÈ©²Xuüøs0Ê¬ŽÑÖ–ô¤Óš•-Üã)è bªáƒ¶xmglpIÅ+ÿ•ã�&§pãƒ�ºg4úï<³éû¨á`‹Å\’cBDënáóE³ÿ
¼:,ûH‡sJ#Gìî½1µoHZœT¥*ŒˆTæR$9¼Êªôß«°v3Çš-Ûö˜¥-"Í]ÖÖ¥òÓ€JMð¡‰È›‹”±gË©wŠŒÊÆÀ¼èþJæÒŒ S»OEÔœ›1í¼5ÙÊ»µLwq|#‘×Š³»Z¯[õl;$�ûî‰ ¥ �Š½åkòåX¾íTä+žUÒ®9ÈS°¸¬´%¾š ùä°™#Qãm¼rˆÇLv·VëøÚ±uñ	h|VîA³_Ú’ÌÑ›ý…ûXªã=5‡‹mÀ%Ö=Qo_z‘(ã 'R94µóPŸÙ–ãë˜–¨†¤ÅžNIÆfH=®²©å„-mêK¦&vº�s&C(ùâˆ�áíE(XÑ4^ùj�	<Ÿ¶@»'ã5ÆÜ]_à¦×Z-4í×iÏ7«ÛæO¿X(ƒQDB‹Ëq KÆZ·9%IÚø #r¤†å€^Þ—íp¡·'„‹Þ¬µä±iPÒ·ÛŽªTÝ�˜Ø¢.²b‘5“fÊ‚§Çäµ”sx†hŽ’ÛEˆ©ÍÇÏœt%Æ8ß€x©–N@+u¥úìOk`à¨q§
»=ÀÊjŠ®%¥3oqTéØ—8)^YÓ.`“Ít"Ž)óR+P€³¨‡‹ãt£_"%ËÐ=.iéo‚²d�á�nøù$¹fkÛò™á�ìápS³Ð¤¬2†çÀri<E\êîQ8,[‰}ƒ‘­Ød ²ò8¼À*õIK/ë,×qùmá¢jbl“uk‚AÜÍCAÿÒê8+Ã5óB›þ›?�öé'ª/•·6FÄ´ÔºAäŒ(>³]J¹ç£Ë¦æÜa"÷0¥Ïªƒ‹¾Ë…¦éõ7ûëÕü'ÐIËœð@·[[Ö%ÚÃfÖØò‘DÑ¹µ¸;¥Y,#öØ×fÉŠÎæ•Î~|J3Z�‹pÛzf<“‘õRgÂ�]‡_p*R¦ÐêÕ’%•°©k`T�mšª§°hóÆ�ÐþsŒ­Íþ¿d<XÀÓX1LŽvì(Õ6+ïÓ7Æ×t=•Â2T2¤¸SeÒ�Êè»*}ˆ!&ÆÚˆc&*E�W‡Œø¶ÿXøŸW…˜qcxÝ/È¦É¶vdn=F‹„”ÌQÂã"MqíSÞf^è¶†ö.3�%ÚË"•ÜZ Ëþ>|¿ÇS…öH#ª’¸~H÷j>ÙCo´Žß¡8¬æg2vØ2-ýÌx+ø€”áÒˆKu +Kjlªéí×äE(ÈÂ$9}‰°$“[Ù$ë_†>–‰N¶
y¼šÀdàAq_£´¿}y4“
UåÞbRì�jO
™Ëƒ'­ŽpK@Mr<í4‚ñä3ýøÂUöh.0×C†âA>(5Ðzu™µ5ØTèSÁKºæC¿h}ÛHˆ'ÿ ´kmh*
åTŒjú !/u¸&…£ñ;€AL£6.êù		�þ8·Z®ê
rÁê`Z÷€I¬à‹šâmþ|“¦&ƒÆ äèðÈ:ðeúk“(Ï$�ª8ÏÿÄÿqÍOò>Ä{NÒÄ…e{WS~XÉp3æ8^ßoÕ£"PmR�+„5˜ÕˆmÙˆ´’aÎé¡b¼sW¼Ë‹ó~jåÚ½Ò#¾ªÊ.A™GK×+Ç`V¨Ù6’Ò9-Ms	ï±²U7¢kâ°ÜŒ´áK`¶'  fn!×3�ê
‰8RuÉ�¡ÅÄ Âƒ-õÅ›¾=}•…™òyã»ðú[ÓøWPèßÑ6‚l2)ãýkõp÷œ>Õít,³]#+Ö|‰|GÿvG s‹ÛBòÝ¾ÔÃÛt“?lt%ÏÛµm¬Ç:ê­*"&¦´œËÿLÒ+3ˆl¼õüÕ×©Ÿš@›‰¹Ù�ÐpÄüðï3±¹"ÞàëÚ—½QþéÁA©·YsúX†ðaû0ª¬@•g¦<âåìlŽ‘£§jÜÀ$	1¯	ËÀó�Ê˜ô=�T_¦#|¦ÍQ»�[ß9w9Ñ&üÛÍ½Ž.ˆ®‘xsÏ_åde¥ŒnV;Üâ{WYn9·¢¡ÃšÅ$£îÏççýµEOùŸ’iëdS*Ý³Ž¹úäFÌØ7fgMûs‡2)�¹�£i�÷9‚V¾’¢% {U©š;	 ÑÈnbÝxÕ4tÃ6:6.?à.Ï	¾TJÈöLgCQBs¢†P°ÖxÁv¡Ëê‡¡_Œ«¥ ü‰üÐºECN´.dÍ…„•6n9^j°jø�™7do}œz¦Â(m¿^îÌ¯‚Q®êH÷¢Ñ5ÈÛC6¥¶VÖí3³l´;å¾;óp÷%¦õþÏødöÿºYoŠw9ìwµh¼¢—Ýøƒ: õË%–óu|àsiUEÓ3•�•6¶òxfïÆ=Î×â?èVýe+AAQ,vŒý<ëè4ÔÃgáÊ0õY\yŒ(<“~9vÇ‹ÄÌÚÍ¨¨j?;z¡P#j~ÛK�é¤­¡ð¶C‰’Ê›h&Fî›/­aª°D7…J¨e0#ä£ …k€+ñ9U‚
,
.‰}7!ÌÌx¨‹@”Í	;ªŠHoü¥K2lîµ�HèzM;\6ƒ‹>�/Wÿ2Ù�½èûZÒpÿ^\|eô#«·†ÚðïŒ¡9òú`q¸ž)ÈV³¹yL–“µ�RáÖ.ˆŒ›7à”Ý#³ãêKŸ¬BÞùAæã“”²Ç"g&Æ'ÀÆê-~tH�÷«/ceôµ¾>òWÎ›/·‚³¨ê­g‡FÞS�sm`ÌxL0hÄ�›aa%.øX7"ìb°í¬¹ŒÈ"GHÕÈ@ù‡^ƒ�Cž"¼=×A ŠX'žYÎ”<mõ³yIÑ[|š=‡utL
9½P"nVlìú^¹™3êÄã†ÔH�ƒ_ÉûoµlÝ`áX.Sœn7Ó±¢ƒx9GË gÑý/`.ú�Û®jv‡�‰LÑI"\@ô3º?×è-}ðvÜ7ët½ÛéD¼y
Ø^Õôåê©IzÀ#òÝÝ°T×ö®�wy¶‘_mw1²�@ƒñõ>áÂ-rf”h¹êy)‹òAv½ˆeXIÕÐ•ÕË§*K‡•ÓìG”<ÖèKWßÏÜ(
c£¡;QG‹“…Ïû…¼N¾dãî?”¯ZwÇŠ2¦–½e?¾m[VHðšÙjèdJd�×kol�M|€ã�äÕÈ+üäóxG�¸+îÒçìPï@zª—äõÞ17l%åãË{Å÷<Š\Èàõ®^lË÷B§:¬‚ŒÛÛWr6ðô¹vTóÎÙD�Ø™Sã;•‡ö>ì)úv ?‹®�­­«‚¤cˆN*¨—tAPLåÿm~äß­ÿ\Øé-öÐÂ4¬Úp0B#?4Ó¡ñŽs13(kg¨ÜÑçBg ÔÒsþý*¨óìÛíÙ„=Ó‘2^,®Ë	x…W6	JNÅèÑ\§XËà¨QÊ|Áén(ÔEP�;¨.óŽÐ×”ßéÄ°Û3«ZJù?1X«H-¨—ã�*]¹°,.ùLXOà‰ŠpY'F2òCC±oÒ–ÄÏNáRt|ó.’æH1|ï!K(_GkJ	
æå@LÚÆÐæ¨yƒ…xá	Kh*ÙÀIÂ;7‚¹ËÛç½€ð� ,ÚðÑ=Ù|Û¶MI¦•àŒ×	ˆÏù~‡õN–Â3Ë4pfüí-€EtË‰ÏQ#>×&À“. ÞašÍæéùºù¨M@§[¥qu¦T�³w>lm+teB~+o‰ª1Þ; é‰ÒÝx×Àð†o…ùÅ	# ›"€²´†ÛÊ»<ŸrAŒì #ÁðÌÊ¿½?ü&õ	ÈÍ?Lìü0D	UšxpõïZzw@œ}±v¼kÒ8cç°b‡@ÏÛ@¼¥O?R]ÆËÐ"s‘X:B^´köŽëõ}ëØ8ÿ^^Ù»,^;{Æ½Ý­;\ùmUˆëê¢âáD;W“s�Ñ¯'Jr‚*ê«ø�Á†˜|¦væ“[¦•@ÒÆ\8ú;üÈ.‘Q%
ˆ)*'�õ;}¦ã¿_³n%~Ùÿ/™¾tÌex˜ŸJÌb%ˆ¬lZÁ FR€Óû¶ ñÇé˜¡äE?ì¥¿u¤©.+ªÄÞ�l�o”«Ô°Œy³n™ª×Ë–½âðÂšu?ø„ƒ!£ÏsÓËj ý#
y^ºH´å†fŽ;�'ªTøhÞŒ‹øTRÀ“L³¾±zä1¿–å«]âªÐ;k@ÓSS7ï“ cüæúÉqÜªu ª:±«ä¾>iQªê·€‡—\Øô›h7¶«™OvsØÖÙª0ÜN¼@¾b%ÂI·ýAÕ_i¯Ì°5Þá.ê­¦Å‘0ÀØ©“_ú·Ýú™Ê¼…h¶´ Êû!ÊŸ‘5„D°�áÈø`ó<vïÊ_ï[Û�ÜS!}„Qh•çý¤Èe=ê0	ùb)k5B”(}N–a¥Òž=îÊ¥äG?Äv°ÞžÜb"#‹–,AÚ´½¼ÿæ˜ô´£ÌmÕ%-Ö ^4‰yUÕ!>ãq0.ÉÆ)9¶ÀõúÌa�‰¾(†éõðê	÷ x™ºàcÇv§ªdŒÒ�^—z;[çÎˆ&N²¬YÍlånUÉ<©BÖù¾ÆâB-�D9rÅƒÆ–ã&@ºÑ|ô˜§å¿Õ®Ôõ††[¡jj˜¯ãÉ"¡„åøM�<(u»éPRçåýw„m tKíºT¼¥ƒw€™^‘þ5K´Ñ³“”‰±}‚¸Åmá\¥)Ö¶ì;F ‚ë=�Æ«ìK;�ßùÃt4£™Dù�\Æ #Avö3}ËT>#ZU»¥èýk6bí“³GI•Ê’cF4sËKR¨Þ_ŽÌë=ã”�£ñÐ(‰—Y˜<^EùÞ¹ÿÒ]é¢¨ÆZœ›�¨{ÄŽ[Þxï�Ý“ýÚ¼Î86
­ð”Í¨ŒëNÿ¨"äÝA™'¸Gˆ4(Pç~›ÞùlÏ/°´Õ!3”‚lOþÒÝe ³Fs'./aÖ%y	üÛ&°qjUjÍs`ð`G:ë—§v1’¸k8Í[ÉpË hÔBØ?ÞqõÒ\¥�’â?M³üâkæ!Ï¿;˜õ1?±¹O\bÖ¤¾Šp»QFÚ×¼*»SŠV¤í…X‡ù£'t<Ý¢Ü$*Þø	¼ÐöñÝV‡pa£:úWB>ŠP³Ðçs­l«!O= «¡SÝž+¶éj‹Bÿõ©
ÌÁ4e¬0Îæã£¾g^'Ûù‚ì±ñ4Ò‰­c•U

URM³s;÷IDÜY$Éå‰3uã¿NÎjùÝÐÁjÒþY_áEEíp©»3þ òÝ—~y4?©–v‡\÷õéz`a�ÜEb­X•DQâXé\„6t1,z²B�,
ßèZÌ§kþ£Í&S@�‘}·�÷ÏÁ˜qéAŸGt‹w¿8^O19Œ¬µ•§�ÓÙ;3NÃð|—i5>nò÷cÁF 4Âß&d.©®M &‚íëóAõþO³&6
ÏFtà˜Àÿ=nÂ•bDÿçû;-qZ*ÎÓƒ#ð‡±Šíù|–	"oûJ	¹Èx~AèšÄÎ»ù½•;–…#bv6ª’íÈéR‡ü ëË-5˜E…Pa…{2ý¥/è¾£¥$°qCÕ{Æ‹ƒóÁ±ztHDµu¿ Œb‡¡g1á#Ìéï *™UvM¼D8ÇìuªÉ»e€¯%ì²é;ì¿Óšæ0 ã¶¶¤«ëzk =qž4wïS{¢òYNÃ1ßžJ–eÔ.ýzK÷Mà¦|l´þE„€¬ .ìGõgwƒà{šY    ùþî÷© þ@Ë€ µšßs±Ägû    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  6<?xml version="1.0"?><Features nextValidID="25"><Feature recentID="0" name="CDH5" directionality="1" translationMW="87530.92" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4764" maxFusedRunOn="4764"><Segment range="2317-4668" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MQRLMMLLATSGACLGLLAVAAVAAAGANPAQRDTHSLLPTHRRQKRDWIWNQMHIDEEKNTSLPHHVGKIKSSVSRKNAKYLLKGEYVGKVFRVDAETGDVFAIERLDRENISEYHLTAVIVDKDTGENLETPSSFTIKVHDVNDNWPVFTHRLFNASVPESSAVGTSVISVTAVDADDPTVGDHASVMYQILKGKEYFAIDNSGRIITITKSLDREKQARYEIVVEARDAQGLRGDSGTATVLVTLQDINDNFPFFTQTKYTFVVPEDTRVGTSVGSLFVEDPDEPQNRMTKYSILRGDYQDAFTIETNPAHNEGIIKPMKPLDYEYIQQYSFIVEATDPTIDLRYMSPPAGNRAQVIINITDVDEPPIFQQPFYHFQLKENQKKPLIGTVLAMDPDAARHSIGYSIRRTSDKGQFFRVTKKGDIYNEKELDREVYPWYNLTVEAKELDSTGTPTGKESIVQVHIEVLDENDNAPEFAKPYQPKVCENAVHGQLVLQISAIDKDITPRNVKFKFILNTENNFTLTDNHDNTANITVKYGQFDREHTKVHFLPVVISDNGMPSRTGTSTLTVAVCKCNEQGEFTFCEDMAAQVGVSIQAVVAILLCILTITVITLLIFLRRRLRKQARAHGKSVPEIHEQLVTYDEEGGGEMDTTSYDVSVLNSVRRGGAKPPRPALDARPSLYAQVQKPPRHAPGAHGGPGEMAAMIEVKKDEADHDGDGPPYDTLHIYGYEGSESIAESLSSLGTDSSDSDVDYDFLNDWGPRFKMLAELYGSDPREELLY"/></Q></Feature><Feature recentID="1" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4764" maxFusedRunOn="4764" detectionMode="exactProteinMatch"><Segment range="4675-4701" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="YPYDVPDYA"/></Q></Feature><Feature recentID="2" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4764" maxFusedRunOn="4764" detectionMode="exactProteinMatch"><Segment range="4705-4731" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="YPYDVPDYA"/></Q></Feature><Feature recentID="3" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4764" maxFusedRunOn="4764" detectionMode="exactProteinMatch"><Segment range="4735-4761" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="YPYDVPDYA"/></Q></Feature><Feature recentID="4" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7233" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6850" maxFusedRunOn="6850" visible="0" detectionMode="exactProteinMatch"><Segment range="7234-7245" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IEGR"/></Q></Feature><Feature recentID="5" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="8066-8082" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="8135-8156" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="8090-8096" color="#ffffff" type="standard"/><Segment range="8097-8114" color="#ffffff" type="standard"/><Segment name="-35" range="8115-8120" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6159" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="6762" maxFusedRunOn="6762" detectionMode="exactProteinMatch"><Segment range="6109-6162" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="EGRGSLLTCGDVEENPGP"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10064-10168" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1804-1921" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7729-7850" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="458-583" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7869-8004" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-414" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2011-2214" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4826-5037" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7424-7657" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1076-1309" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5050-5318" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6763-7350" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="8444-9032" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6163-6762" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"/></Q></Feature><Feature recentID="23" name="CopGFP" directionality="1" translationMW="24523.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="6762" maxFusedRunOn="6762"><Segment range="5377-6039" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGGYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"/></Q></Feature><Feature recentID="24" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="9994" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9203-9994" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="9995-10063" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  °<Notes>
<UUID>dbd6aa57-318e-4875-8005-231b4681e9ac</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pLV3-CMV-CDH5(human)-3Ã—HA-CopGFP-Puro</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.6.7</Created>
<LastModified UTC="1:10:53">2025.9.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      DO  d               E              ÿÿÿÿYô? P                                                                                                                                                             g<StrandColors><TopStrand><ColorRange range="0..2314" colors="black"/><ColorRange range="2315..2329" colors="green"/><ColorRange range="2330..10666" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2314" colors="black"/><ColorRange range="2315..2329" colors="green"/><ColorRange range="2330..10666" colors="black"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
